Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29332 | 3' | -50 | NC_006150.1 | + | 54245 | 0.66 | 0.998498 |
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Target: 5'- aGAAacGCGGgaaaggaauUUUCCUGGACGUGCAc- -3' miRNA: 3'- -CUUacCGCC---------AAGGGAUUUGCACGUaa -5' |
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| 29332 | 3' | -50 | NC_006150.1 | + | 185130 | 0.67 | 0.997151 |
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Target: 5'- aGAUGGCGGUUUCuCUAGcaauauuggugcuugGCG-GCGUUg -3' miRNA: 3'- cUUACCGCCAAGG-GAUU---------------UGCaCGUAA- -5' |
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| 29332 | 3' | -50 | NC_006150.1 | + | 181290 | 0.69 | 0.985301 |
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Target: 5'- ---aGGCGGcugCCCUGGGCGgcgGCGg- -3' miRNA: 3'- cuuaCCGCCaa-GGGAUUUGCa--CGUaa -5' |
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| 29332 | 3' | -50 | NC_006150.1 | + | 58410 | 0.7 | 0.976731 |
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Target: 5'- -uGUGGCGGUaCCCUGAucgGCGaGCGa- -3' miRNA: 3'- cuUACCGCCAaGGGAUU---UGCaCGUaa -5' |
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| 29332 | 3' | -50 | NC_006150.1 | + | 117693 | 0.77 | 0.736303 |
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Target: 5'- aGggUGGCGGUcucgccauUCuCCUGAugGUGCGc- -3' miRNA: 3'- -CuuACCGCCA--------AG-GGAUUugCACGUaa -5' |
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| 29332 | 3' | -50 | NC_006150.1 | + | 160976 | 1.07 | 0.018575 |
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Target: 5'- aGAAUGGCGGUUCCCUAAACGUGCAUUg -3' miRNA: 3'- -CUUACCGCCAAGGGAUUUGCACGUAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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