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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29334 | 3' | -51.5 | NC_006150.1 | + | 124282 | 0.66 | 0.994439 |
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Target: 5'- --aGGGGUGGaaccgaggCCUAUGacgcucggcaacgccCGCGUCAGg -3' miRNA: 3'- uuaCCCCACCa-------GGAUAU---------------GUGCAGUUa -5' |
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| 29334 | 3' | -51.5 | NC_006150.1 | + | 161497 | 0.71 | 0.924737 |
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Target: 5'- cAAUGGGGUGGcccauugcccauUCCcacgcccccUAUugACGUCAAUg -3' miRNA: 3'- -UUACCCCACC------------AGG---------AUAugUGCAGUUA- -5' |
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| 29334 | 3' | -51.5 | NC_006150.1 | + | 193352 | 0.72 | 0.879745 |
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Target: 5'- uGAUGaGGGUGGaUCCUAUACAgG-CAAa -3' miRNA: 3'- -UUAC-CCCACC-AGGAUAUGUgCaGUUa -5' |
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| 29334 | 3' | -51.5 | NC_006150.1 | + | 161287 | 0.77 | 0.635123 |
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Target: 5'- cAAUGGGGcGGUCCUAUG-ACGUUAAUg -3' miRNA: 3'- -UUACCCCaCCAGGAUAUgUGCAGUUA- -5' |
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| 29334 | 3' | -51.5 | NC_006150.1 | + | 161306 | 1.04 | 0.018343 |
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Target: 5'- cAAUGGGGUGGUCCUAUACACGUCAAUg -3' miRNA: 3'- -UUACCCCACCAGGAUAUGUGCAGUUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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