Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
29338 | 3' | -59 | NC_006150.1 | + | 152637 | 0.66 | 0.864748 |
Target: 5'- --cCCCGCaGGCCGUGGC-CGccUUCAa -3' miRNA: 3'- uuaGGGCG-CUGGCACCGaGCacGAGU- -5' |
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29338 | 3' | -59 | NC_006150.1 | + | 122395 | 0.67 | 0.789612 |
Target: 5'- cGUCCagcggCGCGucACCGUGGCUCGggcauuugucaguuUGUUCAg -3' miRNA: 3'- uUAGG-----GCGC--UGGCACCGAGC--------------ACGAGU- -5' |
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29338 | 3' | -59 | NC_006150.1 | + | 106509 | 0.68 | 0.756218 |
Target: 5'- --cCCCGCGGCCGcGGCUguUGCcgUCAg -3' miRNA: 3'- uuaGGGCGCUGGCaCCGAgcACG--AGU- -5' |
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29338 | 3' | -59 | NC_006150.1 | + | 118270 | 0.76 | 0.322497 |
Target: 5'- ---aCCGCGACCGcgGGCUCGagcgGCUCGa -3' miRNA: 3'- uuagGGCGCUGGCa-CCGAGCa---CGAGU- -5' |
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29338 | 3' | -59 | NC_006150.1 | + | 170250 | 1.05 | 0.003647 |
Target: 5'- cAAUCCCGCGACCGUGGCUCGUGCUCAc -3' miRNA: 3'- -UUAGGGCGCUGGCACCGAGCACGAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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