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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29338 | 5' | -53 | NC_006150.1 | + | 184978 | 0.66 | 0.985762 |
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Target: 5'- cUG-GAGgGCGGCaagcugUUcAUGACGGGAUa -3' miRNA: 3'- -ACaCUCgCGCCGga----AA-UACUGCUCUA- -5' |
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| 29338 | 5' | -53 | NC_006150.1 | + | 205283 | 0.67 | 0.972465 |
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Target: 5'- aUG-GAGCGCGGaCCggaacgGAUGAGGg -3' miRNA: 3'- -ACaCUCGCGCC-GGaaaua-CUGCUCUa -5' |
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| 29338 | 5' | -53 | NC_006150.1 | + | 32916 | 0.67 | 0.966495 |
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Target: 5'- aGgcgGAGCGCGGUCggc--GGCGGGGa -3' miRNA: 3'- aCa--CUCGCGCCGGaaauaCUGCUCUa -5' |
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| 29338 | 5' | -53 | NC_006150.1 | + | 128526 | 0.68 | 0.959664 |
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Target: 5'- --aGAGgGCGGCCacauugGUGaACGAGGUa -3' miRNA: 3'- acaCUCgCGCCGGaaa---UAC-UGCUCUA- -5' |
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| 29338 | 5' | -53 | NC_006150.1 | + | 144350 | 0.68 | 0.947714 |
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Target: 5'- --aGGGCuGCGGCCUaaacguUUGUGAUGAuGAUa -3' miRNA: 3'- acaCUCG-CGCCGGA------AAUACUGCU-CUA- -5' |
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| 29338 | 5' | -53 | NC_006150.1 | + | 81407 | 0.7 | 0.911453 |
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Target: 5'- gGUGGGCGCGGCCauucaugGGCaGGAc -3' miRNA: 3'- aCACUCGCGCCGGaaaua--CUGcUCUa -5' |
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| 29338 | 5' | -53 | NC_006150.1 | + | 170284 | 1.06 | 0.011269 |
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Target: 5'- aUGUGAGCGCGGCCUUUAUGACGAGAUc -3' miRNA: 3'- -ACACUCGCGCCGGAAAUACUGCUCUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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