Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29343 | 5' | -53.4 | NC_006150.1 | + | 26488 | 0.66 | 0.98483 |
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Target: 5'- aAUGCCGuCUCCgCGCG-AGcGCAUGg- -3' miRNA: 3'- gUACGGUuGAGG-GCGUaUC-CGUACac -5' |
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| 29343 | 5' | -53.4 | NC_006150.1 | + | 131074 | 0.66 | 0.982968 |
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Target: 5'- gAUGCCAG-UCgaCGCAUAGG-GUGUGg -3' miRNA: 3'- gUACGGUUgAGg-GCGUAUCCgUACAC- -5' |
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| 29343 | 5' | -53.4 | NC_006150.1 | + | 218153 | 0.66 | 0.980939 |
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Target: 5'- gCAUGCCcuCcgCCCGCuu-GGC-UGUGg -3' miRNA: 3'- -GUACGGuuGa-GGGCGuauCCGuACAC- -5' |
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| 29343 | 5' | -53.4 | NC_006150.1 | + | 105712 | 0.66 | 0.978734 |
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Target: 5'- aCAUGgCAGCUCCCGUcuauGUGGGag-GUu -3' miRNA: 3'- -GUACgGUUGAGGGCG----UAUCCguaCAc -5' |
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| 29343 | 5' | -53.4 | NC_006150.1 | + | 161893 | 0.66 | 0.978503 |
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Target: 5'- gGUGCCAAg-UCCGaCAUAuuggauuGGCAUGUGu -3' miRNA: 3'- gUACGGUUgaGGGC-GUAU-------CCGUACAC- -5' |
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| 29343 | 5' | -53.4 | NC_006150.1 | + | 147177 | 0.68 | 0.953909 |
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Target: 5'- aCAUGUCuuugucuuGGCagCCCGCGUuGGCAUGUa -3' miRNA: 3'- -GUACGG--------UUGa-GGGCGUAuCCGUACAc -5' |
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| 29343 | 5' | -53.4 | NC_006150.1 | + | 191495 | 0.68 | 0.949817 |
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Target: 5'- --aGCCG---UCCGCGUGGGCggGUGg -3' miRNA: 3'- guaCGGUugaGGGCGUAUCCGuaCAC- -5' |
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| 29343 | 5' | -53.4 | NC_006150.1 | + | 204017 | 0.69 | 0.920253 |
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Target: 5'- --cGCCGAUUCCCuagcgcaguGCGUGGGCGcGUa -3' miRNA: 3'- guaCGGUUGAGGG---------CGUAUCCGUaCAc -5' |
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| 29343 | 5' | -53.4 | NC_006150.1 | + | 184029 | 1.08 | 0.0074 |
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Target: 5'- cCAUGCCAACUCCCGCAUAGGCAUGUGu -3' miRNA: 3'- -GUACGGUUGAGGGCGUAUCCGUACAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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