Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29344 | 3' | -50.5 | NC_006150.1 | + | 185465 | 0.67 | 0.996157 |
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Target: 5'- aUUgCCGggucauagaagagacAAGGCUugUGGUGCUcauGUGGACa -3' miRNA: 3'- aGAgGGU---------------UUCCGA--ACCACGA---UAUCUG- -5' |
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| 29344 | 3' | -50.5 | NC_006150.1 | + | 105720 | 0.67 | 0.99444 |
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Target: 5'- gCUCCCGucuauguggGAGGUuuuUUGGUGCgcUAcGACg -3' miRNA: 3'- aGAGGGU---------UUCCG---AACCACGauAU-CUG- -5' |
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| 29344 | 3' | -50.5 | NC_006150.1 | + | 25052 | 0.67 | 0.992577 |
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Target: 5'- aUUCCaAAAGGUUagggUGGUGCUGgggcgGGACc -3' miRNA: 3'- aGAGGgUUUCCGA----ACCACGAUa----UCUG- -5' |
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| 29344 | 3' | -50.5 | NC_006150.1 | + | 186582 | 0.68 | 0.991475 |
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Target: 5'- uUC-CCCAGAGGCcaGGcGCcAUAGAUa -3' miRNA: 3'- -AGaGGGUUUCCGaaCCaCGaUAUCUG- -5' |
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| 29344 | 3' | -50.5 | NC_006150.1 | + | 204896 | 0.68 | 0.990247 |
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Target: 5'- cCUCgCCAgGGGGCUUGGgagagGCU--AGACc -3' miRNA: 3'- aGAG-GGU-UUCCGAACCa----CGAuaUCUG- -5' |
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| 29344 | 3' | -50.5 | NC_006150.1 | + | 130925 | 0.68 | 0.988885 |
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Target: 5'- cCUCCauCAAGGGUUUGGUGUgu--GAUg -3' miRNA: 3'- aGAGG--GUUUCCGAACCACGauauCUG- -5' |
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| 29344 | 3' | -50.5 | NC_006150.1 | + | 219081 | 0.69 | 0.981911 |
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Target: 5'- uUUUCCCAuuGGCagUGGUGCUugcuuGAUu -3' miRNA: 3'- -AGAGGGUuuCCGa-ACCACGAuau--CUG- -5' |
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| 29344 | 3' | -50.5 | NC_006150.1 | + | 198003 | 0.7 | 0.958865 |
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Target: 5'- gCUCCUGGAGGCUcaUGGUGCg------ -3' miRNA: 3'- aGAGGGUUUCCGA--ACCACGauaucug -5' |
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| 29344 | 3' | -50.5 | NC_006150.1 | + | 186298 | 1.11 | 0.009485 |
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Target: 5'- cUCUCCCAAAGGCUUGGUGCUAUAGACu -3' miRNA: 3'- -AGAGGGUUUCCGAACCACGAUAUCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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