Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
29346 | 3' | -48 | NC_006150.1 | + | 5779 | 0.67 | 0.999783 |
Target: 5'- uGGUaguagaCGCCACACa-GUUUCGUAAa -3' miRNA: 3'- uCCAaaa---GCGGUGUGgcUAAAGCAUU- -5' |
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29346 | 3' | -48 | NC_006150.1 | + | 122581 | 0.69 | 0.99819 |
Target: 5'- uGGUUccUgGCCACACCacGUUUCGUGg -3' miRNA: 3'- uCCAAa-AgCGGUGUGGc-UAAAGCAUu -5' |
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29346 | 3' | -48 | NC_006150.1 | + | 120831 | 0.72 | 0.981178 |
Target: 5'- cGGUgggUCGUCACGCCGGccgUUGUAGc -3' miRNA: 3'- uCCAaa-AGCGGUGUGGCUaa-AGCAUU- -5' |
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29346 | 3' | -48 | NC_006150.1 | + | 187623 | 1.07 | 0.028475 |
Target: 5'- gGGGUUUUCGCCACACCGAUUUCGUAAg -3' miRNA: 3'- -UCCAAAAGCGGUGUGGCUAAAGCAUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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