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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
29346 | 5' | -45.2 | NC_006150.1 | + | 40426 | 0.66 | 1 |
Target: 5'- ----cUGAUCGGUcagaGCGAUGAGGUa-- -3' miRNA: 3'- gaaauACUAGCCA----CGCUGCUUUAgaa -5' |
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29346 | 5' | -45.2 | NC_006150.1 | + | 197148 | 0.66 | 1 |
Target: 5'- gUUUGUGAUCGGUG-GGCucauGAUUUUg -3' miRNA: 3'- gAAAUACUAGCCACgCUGcu--UUAGAA- -5' |
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29346 | 5' | -45.2 | NC_006150.1 | + | 192698 | 0.66 | 0.999999 |
Target: 5'- ----cUGucuUCGGguucUGCGGCGAAGUCUc -3' miRNA: 3'- gaaauACu--AGCC----ACGCUGCUUUAGAa -5' |
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29346 | 5' | -45.2 | NC_006150.1 | + | 54162 | 0.67 | 0.999995 |
Target: 5'- ---aAUGAaaacCGGUGCG-CGAAAUCa- -3' miRNA: 3'- gaaaUACUa---GCCACGCuGCUUUAGaa -5' |
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29346 | 5' | -45.2 | NC_006150.1 | + | 187661 | 1.08 | 0.052092 |
Target: 5'- aCUUUAUGAUCGGUGCGACGAAAUCUUg -3' miRNA: 3'- -GAAAUACUAGCCACGCUGCUUUAGAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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