Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29349 | 5' | -46.3 | NC_006150.1 | + | 92802 | 0.67 | 0.999992 |
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Target: 5'- --cAGCgCGUAACUAGUucgGUgcGUAGGUCGa -3' miRNA: 3'- gcaUCG-GCAUUGAUCG---CA--CAUUUAGC- -5' |
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| 29349 | 5' | -46.3 | NC_006150.1 | + | 130243 | 0.67 | 0.999984 |
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Target: 5'- uGUAGauuCCGUGGCUAGCaccugugagGUGGAUCu -3' miRNA: 3'- gCAUC---GGCAUUGAUCGca-------CAUUUAGc -5' |
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| 29349 | 5' | -46.3 | NC_006150.1 | + | 115267 | 0.68 | 0.999971 |
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Target: 5'- uCGUccGGCCccGACUAGCGUGcucuGUCa -3' miRNA: 3'- -GCA--UCGGcaUUGAUCGCACauu-UAGc -5' |
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| 29349 | 5' | -46.3 | NC_006150.1 | + | 27483 | 0.68 | 0.999961 |
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Target: 5'- aGUGGCCGccggaUAGCgugAGCGUGcuGAUUGc -3' miRNA: 3'- gCAUCGGC-----AUUGa--UCGCACauUUAGC- -5' |
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| 29349 | 5' | -46.3 | NC_006150.1 | + | 102875 | 0.69 | 0.999882 |
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Target: 5'- uGUAGgCGccuACUGGCGggcaagGUGGGUCGu -3' miRNA: 3'- gCAUCgGCau-UGAUCGCa-----CAUUUAGC- -5' |
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| 29349 | 5' | -46.3 | NC_006150.1 | + | 48881 | 0.69 | 0.99975 |
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Target: 5'- gCGUGGCuCGUA--UAGUGUGUGuuAAUCa -3' miRNA: 3'- -GCAUCG-GCAUugAUCGCACAU--UUAGc -5' |
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| 29349 | 5' | -46.3 | NC_006150.1 | + | 195558 | 0.71 | 0.998765 |
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Target: 5'- aGUGGCUGUGACcgucgccauguacGGCGUGUA--UCGa -3' miRNA: 3'- gCAUCGGCAUUGa------------UCGCACAUuuAGC- -5' |
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| 29349 | 5' | -46.3 | NC_006150.1 | + | 123092 | 0.72 | 0.996641 |
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Target: 5'- uCGUAGCCGUGuuacgauccuaACUGGCcu-UGAAUCGa -3' miRNA: 3'- -GCAUCGGCAU-----------UGAUCGcacAUUUAGC- -5' |
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| 29349 | 5' | -46.3 | NC_006150.1 | + | 196737 | 1.11 | 0.032694 |
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Target: 5'- cCGUAGCCGUAACUAGCGUGUAAAUCGu -3' miRNA: 3'- -GCAUCGGCAUUGAUCGCACAUUUAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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