Results 21 - 27 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
29350 | 5' | -54.4 | NC_006150.1 | + | 101997 | 0.71 | 0.797541 |
Target: 5'- cGCgUGUcCGAGGCGgaGGCGcUGCUCa -3' miRNA: 3'- aCG-ACAuGCUUCGCgaCUGCuGCGAG- -5' |
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29350 | 5' | -54.4 | NC_006150.1 | + | 70296 | 0.72 | 0.788535 |
Target: 5'- aGCUG---GAAGCGCucgUGGCGGCGCUg -3' miRNA: 3'- aCGACaugCUUCGCG---ACUGCUGCGAg -5' |
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29350 | 5' | -54.4 | NC_006150.1 | + | 107262 | 0.73 | 0.731894 |
Target: 5'- gGCUGUGCGGcagauuGCGCcGcCGGCGCUUc -3' miRNA: 3'- aCGACAUGCUu-----CGCGaCuGCUGCGAG- -5' |
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29350 | 5' | -54.4 | NC_006150.1 | + | 202978 | 0.73 | 0.702302 |
Target: 5'- uUGCUGcUGCgGAGGCGgaGGCGACgGCUg -3' miRNA: 3'- -ACGAC-AUG-CUUCGCgaCUGCUG-CGAg -5' |
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29350 | 5' | -54.4 | NC_006150.1 | + | 128699 | 0.76 | 0.560903 |
Target: 5'- cUGCUGUAaCGAGGCGUuccucccccUGAUGACGUUUu -3' miRNA: 3'- -ACGACAU-GCUUCGCG---------ACUGCUGCGAG- -5' |
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29350 | 5' | -54.4 | NC_006150.1 | + | 214918 | 0.78 | 0.428247 |
Target: 5'- cGCUGU-CGGguaggccccacGGCGCUGGCGguGCGCUCg -3' miRNA: 3'- aCGACAuGCU-----------UCGCGACUGC--UGCGAG- -5' |
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29350 | 5' | -54.4 | NC_006150.1 | + | 204712 | 1.09 | 0.005232 |
Target: 5'- cUGCUGUACGAAGCGCUGACGACGCUCu -3' miRNA: 3'- -ACGACAUGCUUCGCGACUGCUGCGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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