Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29353 | 3' | -48.2 | NC_006150.1 | + | 183542 | 0.66 | 0.999944 |
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Target: 5'- uUUAACaCGGUCUcuGGCGUUUGcGCUGc -3' miRNA: 3'- cAAUUG-GCUAGA--UCGCAAACcUGGCu -5' |
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| 29353 | 3' | -48.2 | NC_006150.1 | + | 220590 | 0.67 | 0.999737 |
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Target: 5'- aGUUGGCCaGUUUGGCG-UUGGcACCa- -3' miRNA: 3'- -CAAUUGGcUAGAUCGCaAACC-UGGcu -5' |
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| 29353 | 3' | -48.2 | NC_006150.1 | + | 137576 | 0.68 | 0.999358 |
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Target: 5'- aUUGACUGcgCUccguucuacGGCGUUUGGuCUGAg -3' miRNA: 3'- cAAUUGGCuaGA---------UCGCAAACCuGGCU- -5' |
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| 29353 | 3' | -48.2 | NC_006150.1 | + | 85907 | 0.68 | 0.999108 |
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Target: 5'- ---cACCGAUCUGGCGgcgcuguguacgGcGGCCGGu -3' miRNA: 3'- caauUGGCUAGAUCGCaaa---------C-CUGGCU- -5' |
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| 29353 | 3' | -48.2 | NC_006150.1 | + | 116698 | 0.69 | 0.997111 |
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Target: 5'- --aAAUCGGUCgccccgUGGCGUU-GGGCCGGg -3' miRNA: 3'- caaUUGGCUAG------AUCGCAAaCCUGGCU- -5' |
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| 29353 | 3' | -48.2 | NC_006150.1 | + | 65585 | 0.71 | 0.989103 |
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Target: 5'- -aUGACCGA--UAGgGUUUGGGCCu- -3' miRNA: 3'- caAUUGGCUagAUCgCAAACCUGGcu -5' |
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| 29353 | 3' | -48.2 | NC_006150.1 | + | 205828 | 1.1 | 0.022436 |
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Target: 5'- uGUUAACCGAUCUAGCGUUUGGACCGAu -3' miRNA: 3'- -CAAUUGGCUAGAUCGCAAACCUGGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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