miRNA: 3'- aaGAGauaa-AAGg--UCACUUUGGCUUG- -5'
miRNA: 3'- aAGAGAUaaa--------AGGUC----ACUUUGGCUUg -5'
miRNA: 3'- aAGAGAuaAAAG-GUCACUUUGGCUug -5'
miRNA: 3'- aagagaUAAAAGGUCa-CUUUGGCUUG- -5'
miRNA: 3'- -AAGAGAUAAAAGGUCACUUUGGCUUG- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29357 | 5' | -44.1 | NC_006150.1 | + | 43242 | 0.66 | 1 |
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Target: 5'- -cCUCcaaagUUCgcaGGUGAGGCCGAGCc -3' miRNA: 3'- aaGAGauaa-AAGg--UCACUUUGGCUUG- -5' |
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| 29357 | 5' | -44.1 | NC_006150.1 | + | 48013 | 0.66 | 1 |
|
Target: 5'- gUCUCUGggguccucgccUCCAGaagcUGAGGCCGAGg -3' miRNA: 3'- aAGAGAUaaa--------AGGUC----ACUUUGGCUUg -5' |
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| 29357 | 5' | -44.1 | NC_006150.1 | + | 69192 | 0.73 | 0.998827 |
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Target: 5'- aUCUCU--UUUCaCGGUGGGACCGGu- -3' miRNA: 3'- aAGAGAuaAAAG-GUCACUUUGGCUug -5' |
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| 29357 | 5' | -44.1 | NC_006150.1 | + | 107107 | 0.76 | 0.984606 |
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Target: 5'- -----aGUUUUCCAGggGAAGCCGAGCa -3' miRNA: 3'- aagagaUAAAAGGUCa-CUUUGGCUUG- -5' |
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| 29357 | 5' | -44.1 | NC_006150.1 | + | 215375 | 1.1 | 0.049248 |
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Target: 5'- cUUCUCUAUUUUCCAGUGAAACCGAACa -3' miRNA: 3'- -AAGAGAUAAAAGGUCACUUUGGCUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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