Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29361 | 3' | -63.8 | NC_006150.1 | + | 184836 | 0.71 | 0.331564 |
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Target: 5'- aUCCCaGCCGAaugucaguccgagcuGCAgCGCAacGCCGCCAa -3' miRNA: 3'- cAGGGaCGGCU---------------CGUgGCGU--CGGCGGU- -5' |
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| 29361 | 3' | -63.8 | NC_006150.1 | + | 88401 | 0.71 | 0.328763 |
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Target: 5'- cUCCCUGCgGuGCGCgGaCGGCCGCg- -3' miRNA: 3'- cAGGGACGgCuCGUGgC-GUCGGCGgu -5' |
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| 29361 | 3' | -63.8 | NC_006150.1 | + | 150216 | 0.72 | 0.28886 |
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Target: 5'- -aCCCUGcCCGGGCAggUCGC-GCCGCCc -3' miRNA: 3'- caGGGAC-GGCUCGU--GGCGuCGGCGGu -5' |
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| 29361 | 3' | -63.8 | NC_006150.1 | + | 181103 | 0.72 | 0.28886 |
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Target: 5'- -cCCCUGuCCGAGCgacgccgacGCCGCuguugcucaGGCCGCCu -3' miRNA: 3'- caGGGAC-GGCUCG---------UGGCG---------UCGGCGGu -5' |
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| 29361 | 3' | -63.8 | NC_006150.1 | + | 26860 | 0.73 | 0.276431 |
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Target: 5'- aUCCCg--CGGGUACCGCAaguGCCGCCAc -3' miRNA: 3'- cAGGGacgGCUCGUGGCGU---CGGCGGU- -5' |
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| 29361 | 3' | -63.8 | NC_006150.1 | + | 195291 | 0.75 | 0.205874 |
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Target: 5'- -aUCCUGCaGAGCGCCGCGGCUGUg- -3' miRNA: 3'- caGGGACGgCUCGUGGCGUCGGCGgu -5' |
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| 29361 | 3' | -63.8 | NC_006150.1 | + | 88492 | 0.75 | 0.187529 |
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Target: 5'- -cCCCcGCCaGGGCugCGCGGCCGCUc -3' miRNA: 3'- caGGGaCGG-CUCGugGCGUCGGCGGu -5' |
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| 29361 | 3' | -63.8 | NC_006150.1 | + | 216383 | 1.07 | 0.001126 |
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Target: 5'- gGUCCCUGCCGAGCACCGCAGCCGCCAc -3' miRNA: 3'- -CAGGGACGGCUCGUGGCGUCGGCGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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