Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29364 | 3' | -54.5 | NC_006150.1 | + | 73663 | 0.66 | 0.966379 |
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Target: 5'- cCACAgAGCGgc-GGUcCGCUCUGCGGu- -3' miRNA: 3'- -GUGU-UCGCgaaCCA-GUGAGACGCCuu -5' |
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| 29364 | 3' | -54.5 | NC_006150.1 | + | 103728 | 0.66 | 0.963081 |
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Target: 5'- cCGCGGGgaGUUUGGUCAUUCUccucGCGGu- -3' miRNA: 3'- -GUGUUCg-CGAACCAGUGAGA----CGCCuu -5' |
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| 29364 | 3' | -54.5 | NC_006150.1 | + | 57172 | 0.66 | 0.955823 |
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Target: 5'- uGCAGGUGCUucgcacccUGGUCAC-CgagGCGGu- -3' miRNA: 3'- gUGUUCGCGA--------ACCAGUGaGa--CGCCuu -5' |
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| 29364 | 3' | -54.5 | NC_006150.1 | + | 219011 | 0.67 | 0.93854 |
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Target: 5'- gCGCGAGCGCgaUGGUUGCagaUGCGGc- -3' miRNA: 3'- -GUGUUCGCGa-ACCAGUGag-ACGCCuu -5' |
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| 29364 | 3' | -54.5 | NC_006150.1 | + | 219450 | 0.67 | 0.933625 |
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Target: 5'- -cCAAGCGCUUGuGUCGCgcgcaCUGCGc-- -3' miRNA: 3'- guGUUCGCGAAC-CAGUGa----GACGCcuu -5' |
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| 29364 | 3' | -54.5 | NC_006150.1 | + | 106442 | 0.68 | 0.899076 |
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Target: 5'- gACAAGCGCUUGGUagggguuacCGCUCg--GGAGu -3' miRNA: 3'- gUGUUCGCGAACCA---------GUGAGacgCCUU- -5' |
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| 29364 | 3' | -54.5 | NC_006150.1 | + | 219216 | 1.06 | 0.007189 |
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Target: 5'- aCACAAGCGCUUGGUCACUCUGCGGAAu -3' miRNA: 3'- -GUGUUCGCGAACCAGUGAGACGCCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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