Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29364 | 5' | -52.5 | NC_006150.1 | + | 202979 | 0.66 | 0.990027 |
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Target: 5'- uGCUgCUGCggaGGCGGAgGCGAcGGCUg- -3' miRNA: 3'- -CGAaGACGa--CCGUCU-CGUUcUCGAac -5' |
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| 29364 | 5' | -52.5 | NC_006150.1 | + | 36185 | 0.67 | 0.981653 |
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Target: 5'- gGCUUCgGC-GcGCAGAGCAaaucGGGGgUUGa -3' miRNA: 3'- -CGAAGaCGaC-CGUCUCGU----UCUCgAAC- -5' |
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| 29364 | 5' | -52.5 | NC_006150.1 | + | 92287 | 0.68 | 0.96886 |
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Target: 5'- gGCgggaUGCUGGCGGuGGUggGAGUggUGg -3' miRNA: 3'- -CGaag-ACGACCGUC-UCGuuCUCGa-AC- -5' |
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| 29364 | 5' | -52.5 | NC_006150.1 | + | 151805 | 0.68 | 0.965685 |
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Target: 5'- aCUUgUGCUGGUAGuguuGGCGGGA-CUUGa -3' miRNA: 3'- cGAAgACGACCGUC----UCGUUCUcGAAC- -5' |
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| 29364 | 5' | -52.5 | NC_006150.1 | + | 170412 | 0.68 | 0.965685 |
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Target: 5'- gGCUUCgGCcGGguGAGCGAGgaGGCc-- -3' miRNA: 3'- -CGAAGaCGaCCguCUCGUUC--UCGaac -5' |
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| 29364 | 5' | -52.5 | NC_006150.1 | + | 181633 | 0.71 | 0.867762 |
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Target: 5'- aGCggugcGCUGGCGGAGCGAGaAGCc-- -3' miRNA: 3'- -CGaaga-CGACCGUCUCGUUC-UCGaac -5' |
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| 29364 | 5' | -52.5 | NC_006150.1 | + | 219256 | 1.11 | 0.004917 |
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Target: 5'- gGCUUCUGCUGGCAGAGCAAGAGCUUGg -3' miRNA: 3'- -CGAAGACGACCGUCUCGUUCUCGAAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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