Results 121 - 126 of 126 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29367 | 5' | -61.1 | NC_006151.1 | + | 69961 | 0.73 | 0.279259 |
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Target: 5'- aGCCCcCCgggGCCGCgCgGGCGcUGCAGCg -3' miRNA: 3'- -CGGGuGGa--CGGCG-GaCUGC-ACGUCGa -5' |
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| 29367 | 5' | -61.1 | NC_006151.1 | + | 104284 | 0.73 | 0.266644 |
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Target: 5'- gGCCCGCC-GCUGCa-GGCGcUGCAGCg -3' miRNA: 3'- -CGGGUGGaCGGCGgaCUGC-ACGUCGa -5' |
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| 29367 | 5' | -61.1 | NC_006151.1 | + | 39018 | 0.74 | 0.254494 |
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Target: 5'- cGCuCCGCCgccgcGCCGCCUGGa-UGCGGCa -3' miRNA: 3'- -CG-GGUGGa----CGGCGGACUgcACGUCGa -5' |
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| 29367 | 5' | -61.1 | NC_006151.1 | + | 124118 | 0.77 | 0.152801 |
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Target: 5'- uGCCCGCC-GCCGuCCggcuccacgGugGUGCGGCUg -3' miRNA: 3'- -CGGGUGGaCGGC-GGa--------CugCACGUCGA- -5' |
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| 29367 | 5' | -61.1 | NC_006151.1 | + | 118713 | 0.79 | 0.109881 |
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Target: 5'- gGCCCGCuCUGCCGCCUGcugcacggcuACGUGCugGGCc -3' miRNA: 3'- -CGGGUG-GACGGCGGAC----------UGCACG--UCGa -5' |
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| 29367 | 5' | -61.1 | NC_006151.1 | + | 140507 | 1.09 | 0.000756 |
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Target: 5'- cGCCCACCUGCCGCCUGACGUGCAGCUa -3' miRNA: 3'- -CGGGUGGACGGCGGACUGCACGUCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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