Results 121 - 126 of 126 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 29367 | 5' | -61.1 | NC_006151.1 | + | 138577 | 0.7 | 0.440106 |
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Target: 5'- cGCCCGCCUGCCccacCCcGuCGUGUAcGCg -3' miRNA: 3'- -CGGGUGGACGGc---GGaCuGCACGU-CGa -5' |
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| 29367 | 5' | -61.1 | NC_006151.1 | + | 138759 | 0.7 | 0.403061 |
|
Target: 5'- cGCCUACCUGUgggacgugcacuuuCGCCUGGCGgccgucgcGUGGCUc -3' miRNA: 3'- -CGGGUGGACG--------------GCGGACUGCa-------CGUCGA- -5' |
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| 29367 | 5' | -61.1 | NC_006151.1 | + | 139672 | 0.67 | 0.562316 |
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Target: 5'- cGCCCGacaggcCCUGCgGCagcGGCGgcgGCAGCa -3' miRNA: 3'- -CGGGU------GGACGgCGga-CUGCa--CGUCGa -5' |
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| 29367 | 5' | -61.1 | NC_006151.1 | + | 140022 | 0.72 | 0.299065 |
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Target: 5'- gGCCgACCUGCacCGCCuguuUGAgGUGCGGCc -3' miRNA: 3'- -CGGgUGGACG--GCGG----ACUgCACGUCGa -5' |
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| 29367 | 5' | -61.1 | NC_006151.1 | + | 140295 | 0.71 | 0.372812 |
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Target: 5'- cGUgCGCCggcGCCGCCUgcggGACGUGC-GCUg -3' miRNA: 3'- -CGgGUGGa--CGGCGGA----CUGCACGuCGA- -5' |
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| 29367 | 5' | -61.1 | NC_006151.1 | + | 140507 | 1.09 | 0.000756 |
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Target: 5'- cGCCCACCUGCCGCCUGACGUGCAGCUa -3' miRNA: 3'- -CGGGUGGACGGCGGACUGCACGUCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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