Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
29386 | 3' | -59.6 | NC_006151.1 | + | 82657 | 0.66 | 0.715132 |
Target: 5'- cGAGGGCgGCccACCCUGcGGcgcgccgcgGGCGAGGg -3' miRNA: 3'- -UUCCUGgCG--UGGGACaCC---------UCGCUCUa -5' |
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29386 | 3' | -59.6 | NC_006151.1 | + | 44342 | 0.66 | 0.715132 |
Target: 5'- gGAGGGCCGCGgaCggGUGGgacGGCGGGGg -3' miRNA: 3'- -UUCCUGGCGUggGa-CACC---UCGCUCUa -5' |
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29386 | 3' | -59.6 | NC_006151.1 | + | 3402 | 0.66 | 0.705148 |
Target: 5'- gGAGGGCCGCGgCgUGUGGgucucgccGGcCGGGAc -3' miRNA: 3'- -UUCCUGGCGUgGgACACC--------UC-GCUCUa -5' |
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29386 | 3' | -59.6 | NC_006151.1 | + | 8130 | 0.66 | 0.705148 |
Target: 5'- -cGGACCcCGCCCgGUGGgggGGCGGGu- -3' miRNA: 3'- uuCCUGGcGUGGGaCACC---UCGCUCua -5' |
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29386 | 3' | -59.6 | NC_006151.1 | + | 89150 | 0.66 | 0.674861 |
Target: 5'- -cGaGACCGCGCCCgccucGGGCGAGGc -3' miRNA: 3'- uuC-CUGGCGUGGGacac-CUCGCUCUa -5' |
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29386 | 3' | -59.6 | NC_006151.1 | + | 49669 | 0.67 | 0.634029 |
Target: 5'- -cGGGCCGCGCCCgGaGGAcGCG-GAc -3' miRNA: 3'- uuCCUGGCGUGGGaCaCCU-CGCuCUa -5' |
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29386 | 3' | -59.6 | NC_006151.1 | + | 89727 | 0.67 | 0.634029 |
Target: 5'- --cGGCCGCGgCCgcgGUGGugGGCGAGAc -3' miRNA: 3'- uucCUGGCGUgGGa--CACC--UCGCUCUa -5' |
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29386 | 3' | -59.6 | NC_006151.1 | + | 77542 | 0.67 | 0.603358 |
Target: 5'- -uGGGCCGC-CCCgcaggGGAGCGAcGAc -3' miRNA: 3'- uuCCUGGCGuGGGaca--CCUCGCU-CUa -5' |
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29386 | 3' | -59.6 | NC_006151.1 | + | 27694 | 0.68 | 0.583005 |
Target: 5'- -cGGucCCGCucgcguCCCUGUGGGGgGGGGg -3' miRNA: 3'- uuCCu-GGCGu-----GGGACACCUCgCUCUa -5' |
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29386 | 3' | -59.6 | NC_006151.1 | + | 130920 | 0.68 | 0.562794 |
Target: 5'- cGGuGGCCGgGCCCgcgGGGGCGGGGg -3' miRNA: 3'- uUC-CUGGCgUGGGacaCCUCGCUCUa -5' |
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29386 | 3' | -59.6 | NC_006151.1 | + | 64866 | 0.7 | 0.474874 |
Target: 5'- gGAGGGCCGCugCCcGgcgGGAGCaGGGc -3' miRNA: 3'- -UUCCUGGCGugGGaCa--CCUCGcUCUa -5' |
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29386 | 3' | -59.6 | NC_006151.1 | + | 125421 | 1.04 | 0.002335 |
Target: 5'- cAAGGACCGCACCCUGUGGAGCGAGAUg -3' miRNA: 3'- -UUCCUGGCGUGGGACACCUCGCUCUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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