Results 41 - 45 of 45 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio![]() |
P value |
Predicted miRNA align pattern | |||||||
29398 | 3' | -57.1 | NC_006151.1 | + | 12759 | 0.66 | 0.825512 |
Target: 5'- gCGgaGGGCUg---GCUcGAGGCcAGCGCg -3' miRNA: 3'- -GCgaCCCGAaggaCGA-CUUCG-UCGUG- -5' |
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29398 | 3' | -57.1 | NC_006151.1 | + | 2327 | 0.66 | 0.825512 |
Target: 5'- aGCgGGGCggCCUcggcgucggGCUccAGCAGCGCc -3' miRNA: 3'- gCGaCCCGaaGGA---------CGAcuUCGUCGUG- -5' |
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29398 | 3' | -57.1 | NC_006151.1 | + | 46591 | 0.66 | 0.816803 |
Target: 5'- ---cGGGCggCCgUGCUGAGGCgcgAGCAg -3' miRNA: 3'- gcgaCCCGaaGG-ACGACUUCG---UCGUg -5' |
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29398 | 3' | -57.1 | NC_006151.1 | + | 115737 | 0.66 | 0.796127 |
Target: 5'- aGUUGcacaccaccuugauGGCcgCCUGCUGcuguccAGCAGCACg -3' miRNA: 3'- gCGAC--------------CCGaaGGACGACu-----UCGUCGUG- -5' |
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29398 | 3' | -57.1 | NC_006151.1 | + | 141616 | 0.66 | 0.83404 |
Target: 5'- gGCUgccgggaucGGGCgggUCgCUGCcGcGGCGGCGCg -3' miRNA: 3'- gCGA---------CCCGa--AG-GACGaCuUCGUCGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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