Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 29398 | 3' | -57.1 | NC_006151.1 | + | 121061 | 0.69 | 0.670506 |
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Target: 5'- gCGCgUGGGCUUCacgUGCUcgcaGAcgggcGGCGGCGCc -3' miRNA: 3'- -GCG-ACCCGAAGg--ACGA----CU-----UCGUCGUG- -5' |
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| 29398 | 3' | -57.1 | NC_006151.1 | + | 123036 | 0.7 | 0.587409 |
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Target: 5'- gGCgUGGGCggaCCUGCcgGccGCGGCGCu -3' miRNA: 3'- gCG-ACCCGaa-GGACGa-CuuCGUCGUG- -5' |
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| 29398 | 3' | -57.1 | NC_006151.1 | + | 130472 | 0.67 | 0.751478 |
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Target: 5'- uGCaGGGCgauggCCUGCc---GCAGCGCg -3' miRNA: 3'- gCGaCCCGaa---GGACGacuuCGUCGUG- -5' |
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| 29398 | 3' | -57.1 | NC_006151.1 | + | 137014 | 0.67 | 0.770829 |
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Target: 5'- gCGCUGGGCgcCCUGCUcGGccuGcCGGaCGCg -3' miRNA: 3'- -GCGACCCGaaGGACGA-CUu--C-GUC-GUG- -5' |
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| 29398 | 3' | -57.1 | NC_006151.1 | + | 141616 | 0.66 | 0.83404 |
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Target: 5'- gGCUgccgggaucGGGCgggUCgCUGCcGcGGCGGCGCg -3' miRNA: 3'- gCGA---------CCCGa--AG-GACGaCuUCGUCGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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