Results 41 - 44 of 44 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
29408 | 3' | -56.6 | NC_006151.1 | + | 81586 | 0.74 | 0.4435 |
Target: 5'- uCUCgcgCgcgUCGccGGGGCCgCCGGCGCGCAg -3' miRNA: 3'- -GAGaa-Ga--AGC--UCCUGG-GGCUGCGCGU- -5' |
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29408 | 3' | -56.6 | NC_006151.1 | + | 126452 | 0.76 | 0.374838 |
Target: 5'- cCUCgagagCGAGGACCCCGACGCc-- -3' miRNA: 3'- -GAGaagaaGCUCCUGGGGCUGCGcgu -5' |
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29408 | 3' | -56.6 | NC_006151.1 | + | 84567 | 0.84 | 0.114079 |
Target: 5'- -gCUUCagCGAGGGCCUCGACGCGCGc -3' miRNA: 3'- gaGAAGaaGCUCCUGGGGCUGCGCGU- -5' |
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29408 | 3' | -56.6 | NC_006151.1 | + | 114351 | 1.09 | 0.002735 |
Target: 5'- gCUCUUCUUCGAGGACCCCGACGCGCAg -3' miRNA: 3'- -GAGAAGAAGCUCCUGGGGCUGCGCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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