Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29408 | 5' | -51.8 | NC_006151.1 | + | 54616 | 0.66 | 0.98779 |
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Target: 5'- --gGUGCGccgGGcGcgCCCCGGGggGa -3' miRNA: 3'- cuaCAUGCa--CCaCaaGGGGCUCuuCa -5' |
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| 29408 | 5' | -51.8 | NC_006151.1 | + | 41092 | 0.66 | 0.986166 |
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Target: 5'- cGGUGUugGUGGUcccGgcgggUCCUggUGGGAGGUa -3' miRNA: 3'- -CUACAugCACCA---Ca----AGGG--GCUCUUCA- -5' |
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| 29408 | 5' | -51.8 | NC_006151.1 | + | 122325 | 0.67 | 0.97546 |
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Target: 5'- cGGUGcGCGUGGa---CCCCGAGGAcGUg -3' miRNA: 3'- -CUACaUGCACCacaaGGGGCUCUU-CA- -5' |
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| 29408 | 5' | -51.8 | NC_006151.1 | + | 99477 | 0.67 | 0.97546 |
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Target: 5'- --cGgcgGCGcUGGgGcUCCCCGAGAAGg -3' miRNA: 3'- cuaCa--UGC-ACCaCaAGGGGCUCUUCa -5' |
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| 29408 | 5' | -51.8 | NC_006151.1 | + | 122220 | 0.67 | 0.963289 |
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Target: 5'- --cGUGCGcGGUGUgCUUCGAGGAGc -3' miRNA: 3'- cuaCAUGCaCCACAaGGGGCUCUUCa -5' |
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| 29408 | 5' | -51.8 | NC_006151.1 | + | 3616 | 0.68 | 0.942857 |
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Target: 5'- cGGUGgcCGUGGccgUCCCCGcGGAGGg -3' miRNA: 3'- -CUACauGCACCacaAGGGGC-UCUUCa -5' |
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| 29408 | 5' | -51.8 | NC_006151.1 | + | 44893 | 0.7 | 0.88317 |
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Target: 5'- cGGUGUGCgGUGGUGUgugUgCgCGAGAGGg -3' miRNA: 3'- -CUACAUG-CACCACA---AgGgGCUCUUCa -5' |
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| 29408 | 5' | -51.8 | NC_006151.1 | + | 136206 | 0.71 | 0.849217 |
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Target: 5'- --gGUACGUGGcgcgcuucggCCCCGAGGAGa -3' miRNA: 3'- cuaCAUGCACCacaa------GGGGCUCUUCa -5' |
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| 29408 | 5' | -51.8 | NC_006151.1 | + | 41337 | 0.71 | 0.844258 |
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Target: 5'- -uUGUugGUGGUGguggUCCCGGGuGGUc -3' miRNA: 3'- cuACAugCACCACaa--GGGGCUCuUCA- -5' |
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| 29408 | 5' | -51.8 | NC_006151.1 | + | 114389 | 1.07 | 0.009331 |
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Target: 5'- uGAUGUACGUGGUGUUCCCCGAGAAGUc -3' miRNA: 3'- -CUACAUGCACCACAAGGGGCUCUUCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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