miRNA: 3'- -GCAGAGGU--CU-------UUGUGGU----------GCGCCGGGa -5'
miRNA: 3'- -GCAgAGGU-CUUuGUGGUGCG---CCGGga -5'
miRNA: 3'- gcAGa--GGUCUU---UGUGGUGcGCCGGGa -5'
miRNA: 3'- -GCAGaGGU-------------CuuUGUG-GUG-CGCCGGGa -5'
Results 101 - 104 of 104 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 29449 | 3' | -56.9 | NC_006151.1 | + | 87582 | 0.66 | 0.842761 |
|
Target: 5'- aCGUCUCCGccGGcaccaucGACGCCAgcucggccgcCGUGGCCUg -3' miRNA: 3'- -GCAGAGGU--CU-------UUGUGGU----------GCGCCGGGa -5' |
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| 29449 | 3' | -56.9 | NC_006151.1 | + | 135349 | 0.67 | 0.835434 |
|
Target: 5'- aCGUgUCCGcGAGcCGCCGCGCguuGGCCg- -3' miRNA: 3'- -GCAgAGGU-CUUuGUGGUGCG---CCGGga -5' |
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| 29449 | 3' | -56.9 | NC_006151.1 | + | 109795 | 0.67 | 0.835434 |
|
Target: 5'- -cUCccgCCGGAGuuuGCGCCGC-CGGCCCc -3' miRNA: 3'- gcAGa--GGUCUU---UGUGGUGcGCCGGGa -5' |
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| 29449 | 3' | -56.9 | NC_006151.1 | + | 78709 | 0.68 | 0.791272 |
|
Target: 5'- uCGUCgCCGcgacggcgcccguGggGCGCgCGCuGCGGCCCa -3' miRNA: 3'- -GCAGaGGU-------------CuuUGUG-GUG-CGCCGGGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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