Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29450 | 5' | -55.3 | NC_006151.1 | + | 24027 | 0.66 | 0.894853 |
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Target: 5'- aCGUUGCCCaCUUCauugUUgaGCUGUGGAGa -3' miRNA: 3'- -GCGAUGGG-GAAGa---AGgaCGACGUCUU- -5' |
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| 29450 | 5' | -55.3 | NC_006151.1 | + | 132421 | 0.66 | 0.880792 |
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Target: 5'- aGCUugCCCUcgUCgcggcgcgCCUGCgcgUGCAGGu -3' miRNA: 3'- gCGAugGGGA--AGaa------GGACG---ACGUCUu -5' |
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| 29450 | 5' | -55.3 | NC_006151.1 | + | 92048 | 0.66 | 0.865794 |
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Target: 5'- cCGCUGCCUg--CUgcgCCUGCUGCGc-- -3' miRNA: 3'- -GCGAUGGGgaaGAa--GGACGACGUcuu -5' |
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| 29450 | 5' | -55.3 | NC_006151.1 | + | 43435 | 0.66 | 0.865794 |
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Target: 5'- gGC-ACCCUUUCUUCgaGUgaUGCAGGu -3' miRNA: 3'- gCGaUGGGGAAGAAGgaCG--ACGUCUu -5' |
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| 29450 | 5' | -55.3 | NC_006151.1 | + | 118695 | 0.67 | 0.836583 |
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Target: 5'- cCGC-GCCCCUucuucaacggcccgcUCUgccgCCUGCUGCAc-- -3' miRNA: 3'- -GCGaUGGGGA---------------AGAa---GGACGACGUcuu -5' |
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| 29450 | 5' | -55.3 | NC_006151.1 | + | 140188 | 0.68 | 0.796526 |
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Target: 5'- aCGCUggACCCCaUCguggCCaccgcgggggcggUGCUGCAGAAc -3' miRNA: 3'- -GCGA--UGGGGaAGaa--GG-------------ACGACGUCUU- -5' |
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| 29450 | 5' | -55.3 | NC_006151.1 | + | 86279 | 0.68 | 0.766052 |
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Target: 5'- cCGCgGCCCCUUCUUCCucggcgucgucaacUGCccGCAGc- -3' miRNA: 3'- -GCGaUGGGGAAGAAGG--------------ACGa-CGUCuu -5' |
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| 29450 | 5' | -55.3 | NC_006151.1 | + | 47086 | 0.7 | 0.677615 |
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Target: 5'- gGCUcccgGCCCCgcaCUcaccuccgcUCCUGCUGCGGGu -3' miRNA: 3'- gCGA----UGGGGaa-GA---------AGGACGACGUCUu -5' |
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| 29450 | 5' | -55.3 | NC_006151.1 | + | 71115 | 1.06 | 0.003554 |
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Target: 5'- gCGCUACCCCUUCUUCCUGCUGCAGAAg -3' miRNA: 3'- -GCGAUGGGGAAGAAGGACGACGUCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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