Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29462 | 5' | -50.9 | NC_006151.1 | + | 97829 | 0.66 | 0.990207 |
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Target: 5'- cGGUaGUUGGUCGucacGUGCgcgcgcagcgcgUCGUCCGCg -3' miRNA: 3'- aUCAaCAACUAGUc---CACG------------AGCAGGUG- -5' |
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| 29462 | 5' | -50.9 | NC_006151.1 | + | 76319 | 0.66 | 0.988804 |
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Target: 5'- gUAGUUGUgcacgggcUGGUCggccGGGUGCggccCGUCgCGCg -3' miRNA: 3'- -AUCAACA--------ACUAG----UCCACGa---GCAG-GUG- -5' |
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| 29462 | 5' | -50.9 | NC_006151.1 | + | 142158 | 0.66 | 0.988804 |
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Target: 5'- -------gGcgCAGGggGCUCGUCCACc -3' miRNA: 3'- aucaacaaCuaGUCCa-CGAGCAGGUG- -5' |
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| 29462 | 5' | -50.9 | NC_006151.1 | + | 83379 | 0.67 | 0.971318 |
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Target: 5'- cAGgcGccg--CAGGUGCUCGUCCGg -3' miRNA: 3'- aUCaaCaacuaGUCCACGAGCAGGUg -5' |
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| 29462 | 5' | -50.9 | NC_006151.1 | + | 24356 | 0.68 | 0.949088 |
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Target: 5'- ----cGUUGAUCGcGG-GCUCGUCCcCg -3' miRNA: 3'- aucaaCAACUAGU-CCaCGAGCAGGuG- -5' |
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| 29462 | 5' | -50.9 | NC_006151.1 | + | 53073 | 0.71 | 0.869001 |
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Target: 5'- aAGcUGUcgacgagGcgCAGGUGCUCGUCCGu -3' miRNA: 3'- aUCaACAa------CuaGUCCACGAGCAGGUg -5' |
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| 29462 | 5' | -50.9 | NC_006151.1 | + | 88484 | 0.75 | 0.68433 |
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Target: 5'- cGGUgcUGUUGGucacgaaggcggcgUCcaccGGGUGCUCGUCCGCg -3' miRNA: 3'- aUCA--ACAACU--------------AG----UCCACGAGCAGGUG- -5' |
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| 29462 | 5' | -50.9 | NC_006151.1 | + | 60991 | 1.09 | 0.006594 |
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Target: 5'- gUAGUUGUUGAUCAGGUGCUCGUCCACg -3' miRNA: 3'- -AUCAACAACUAGUCCACGAGCAGGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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