Results 21 - 23 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position![]() |
R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
29471 | 3' | -54 | NC_006151.1 | + | 21475 | 0.66 | 0.941885 |
Target: 5'- gCGUCgcCGGCUCGGgcaggcggccgggggGCGCgggcgUCACCGg -3' miRNA: 3'- -GUAGauGUUGAGCCa--------------CGCGa----GGUGGC- -5' |
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29471 | 3' | -54 | NC_006151.1 | + | 20747 | 0.68 | 0.882166 |
Target: 5'- ----aGCAGC-CGGcGCGcCUCCACCGc -3' miRNA: 3'- guagaUGUUGaGCCaCGC-GAGGUGGC- -5' |
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29471 | 3' | -54 | NC_006151.1 | + | 20094 | 0.67 | 0.936593 |
Target: 5'- uCAUCUugGGCUgGGggacggGCGCcCCGgCGa -3' miRNA: 3'- -GUAGAugUUGAgCCa-----CGCGaGGUgGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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