Results 61 - 65 of 65 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29471 | 5' | -58.2 | NC_006151.1 | + | 57447 | 0.66 | 0.781544 |
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Target: 5'- cGCGGCGGcccccgcgucggCCAGgugCCGCGCcGCg- -3' miRNA: 3'- aCGCCGUCua----------GGUCa--GGUGCGaCGag -5' |
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| 29471 | 5' | -58.2 | NC_006151.1 | + | 131914 | 0.66 | 0.785241 |
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Target: 5'- gGCGGCAGcagggCC--UCCGCGgUGCUg -3' miRNA: 3'- aCGCCGUCua---GGucAGGUGCgACGAg -5' |
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| 29471 | 5' | -58.2 | NC_006151.1 | + | 4632 | 0.66 | 0.785241 |
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Target: 5'- cGCuGGCAGAaCUGGUUgaaGCGCUGgUCg -3' miRNA: 3'- aCG-CCGUCUaGGUCAGg--UGCGACgAG- -5' |
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| 29471 | 5' | -58.2 | NC_006151.1 | + | 21335 | 0.66 | 0.785241 |
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Target: 5'- cGCGGCGGG---GGUCCGCgGCUggaacgGCUCc -3' miRNA: 3'- aCGCCGUCUaggUCAGGUG-CGA------CGAG- -5' |
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| 29471 | 5' | -58.2 | NC_006151.1 | + | 52395 | 0.65 | 0.8007 |
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Target: 5'- gGCGGCGGGcgCCg--CCGCGCccgagaagucaaagUGCUCc -3' miRNA: 3'- aCGCCGUCUa-GGucaGGUGCG--------------ACGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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