miRNA: 3'- aCGcCGUCUAGGUcaGGUGCGa--CG-AG- -5'
miRNA: 3'- aCGCCGUCua---GGucAGGUGCgACGAg -5'
miRNA: 3'- aCGCCGUCUaGGUCaGGUGCGa-CGag -5'
miRNA: 3'- aCGCCGUC-----UAGGUCag--GUGCGa----CGAG- -5'
miRNA: 3'- aCGCCGUcuaGGUCAGGUGCga-------CGAG- -5'
Results 61 - 65 of 65 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 29471 | 5' | -58.2 | NC_006151.1 | + | 131660 | 0.66 | 0.757014 |
|
Target: 5'- cGCcGCAGAUCCGa-CCGCGCgcgGCgUCa -3' miRNA: 3'- aCGcCGUCUAGGUcaGGUGCGa--CG-AG- -5' |
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| 29471 | 5' | -58.2 | NC_006151.1 | + | 131914 | 0.66 | 0.785241 |
|
Target: 5'- gGCGGCAGcagggCC--UCCGCGgUGCUg -3' miRNA: 3'- aCGCCGUCua---GGucAGGUGCgACGAg -5' |
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| 29471 | 5' | -58.2 | NC_006151.1 | + | 132124 | 0.7 | 0.515343 |
|
Target: 5'- aGCGGCAGGcCCAGcUCGCGCcgGCg- -3' miRNA: 3'- aCGCCGUCUaGGUCaGGUGCGa-CGag -5' |
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| 29471 | 5' | -58.2 | NC_006151.1 | + | 132470 | 0.68 | 0.677541 |
|
Target: 5'- cGCGGCAGuaggcGUCCAGcaggCGCGCguagcGCUCc -3' miRNA: 3'- aCGCCGUC-----UAGGUCag--GUGCGa----CGAG- -5' |
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| 29471 | 5' | -58.2 | NC_006151.1 | + | 139563 | 0.7 | 0.555059 |
|
Target: 5'- aGCGGCGc--CCGGUCCGCGagcgccuggGCUCg -3' miRNA: 3'- aCGCCGUcuaGGUCAGGUGCga-------CGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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