Results 61 - 65 of 65 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 29471 | 5' | -58.2 | NC_006151.1 | + | 4282 | 0.68 | 0.657117 |
|
Target: 5'- cGCGGCAGG-CCAggacgcaggcGUCCGC-CaGCUCg -3' miRNA: 3'- aCGCCGUCUaGGU----------CAGGUGcGaCGAG- -5' |
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| 29471 | 5' | -58.2 | NC_006151.1 | + | 4048 | 0.69 | 0.605849 |
|
Target: 5'- cGCGggcGCGGGcCCcGUCCACGCUGUa- -3' miRNA: 3'- aCGC---CGUCUaGGuCAGGUGCGACGag -5' |
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| 29471 | 5' | -58.2 | NC_006151.1 | + | 3437 | 0.66 | 0.775958 |
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Target: 5'- cGCGGCGGAagCCGccGUCgGgCGCggggUGCUCg -3' miRNA: 3'- aCGCCGUCUa-GGU--CAGgU-GCG----ACGAG- -5' |
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| 29471 | 5' | -58.2 | NC_006151.1 | + | 2609 | 0.7 | 0.545036 |
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Target: 5'- gGCGGCAGcgggaccgggGUCCGGggCCcgGCGCgggUGCUCa -3' miRNA: 3'- aCGCCGUC----------UAGGUCa-GG--UGCG---ACGAG- -5' |
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| 29471 | 5' | -58.2 | NC_006151.1 | + | 2386 | 0.68 | 0.667344 |
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Target: 5'- gGcCGGCAGGcccUCgGGgCCGCGgaGCUCg -3' miRNA: 3'- aC-GCCGUCU---AGgUCaGGUGCgaCGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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