Results 41 - 43 of 43 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position![]() |
R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
29489 | 5' | -44.8 | NC_006151.1 | + | 2769 | 0.67 | 0.999963 |
Target: 5'- cCAGCAc---GGCCGCGCgGA-GCUCg -3' miRNA: 3'- -GUUGUuuuuUCGGUGCGaCUaUGAGa -5' |
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29489 | 5' | -44.8 | NC_006151.1 | + | 2613 | 0.71 | 0.997678 |
Target: 5'- gCAGCGGGAccgggguccgGGGCCcgGCGCgGGUGCUCa -3' miRNA: 3'- -GUUGUUUU----------UUCGG--UGCGaCUAUGAGa -5' |
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29489 | 5' | -44.8 | NC_006151.1 | + | 2473 | 0.67 | 0.99995 |
Target: 5'- cCAGCGGuu-GGCCGCGC-GGUGCcCg -3' miRNA: 3'- -GUUGUUuuuUCGGUGCGaCUAUGaGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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