miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
29491 5' -59.4 NC_006151.1 + 139360 0.66 0.733902
Target:  5'- cUCU-GCCUG-CUGGUAGG-GGG-CGu -3'
miRNA:   3'- aAGAuCGGACgGACCAUCCgCCCaGC- -5'
29491 5' -59.4 NC_006151.1 + 136977 0.66 0.724095
Target:  5'- -gCUGGCCgccgcGCC-GGgcGGCGGG-CGc -3'
miRNA:   3'- aaGAUCGGa----CGGaCCauCCGCCCaGC- -5'
29491 5' -59.4 NC_006151.1 + 27355 0.68 0.612982
Target:  5'- gUCgugGGCCgggGCCc---GGGCGGGUCGa -3'
miRNA:   3'- aAGa--UCGGa--CGGaccaUCCGCCCAGC- -5'
29491 5' -59.4 NC_006151.1 + 82872 0.69 0.552392
Target:  5'- aUCUGGCCgcgGCgCgGGUGGGaggggcgaGGGUCa -3'
miRNA:   3'- aAGAUCGGa--CG-GaCCAUCCg-------CCCAGc -5'
29491 5' -59.4 NC_006151.1 + 141614 0.69 0.522742
Target:  5'- ---gAGgCUGCCgGGaucGGGCGGGUCGc -3'
miRNA:   3'- aagaUCgGACGGaCCa--UCCGCCCAGC- -5'
29491 5' -59.4 NC_006151.1 + 43838 1.07 0.001571
Target:  5'- cUUCUAGCCUGCCUGGUAGGCGGGUCGg -3'
miRNA:   3'- -AAGAUCGGACGGACCAUCCGCCCAGC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.