Results 121 - 128 of 128 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 29494 | 3' | -58.5 | NC_006151.1 | + | 33460 | 0.68 | 0.654178 |
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Target: 5'- gAGAGAGAGGAGAgGCGagagaGGaGGAGaaggaggaGGCGa -3' miRNA: 3'- -UCUCUCUUCUCUaCGC-----CC-CCUC--------CCGC- -5' |
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| 29494 | 3' | -58.5 | NC_006151.1 | + | 79291 | 0.69 | 0.64407 |
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Target: 5'- cGGAGAGGAGAGucccGCGccccGGGAcGGGCc -3' miRNA: 3'- -UCUCUCUUCUCua--CGCc---CCCU-CCCGc -5' |
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| 29494 | 3' | -58.5 | NC_006151.1 | + | 26256 | 0.69 | 0.64407 |
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Target: 5'- cAGAGGGGAGGGGga-GaGGGGcaaacgauGGGGCGg -3' miRNA: 3'- -UCUCUCUUCUCUacgC-CCCC--------UCCCGC- -5' |
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| 29494 | 3' | -58.5 | NC_006151.1 | + | 8210 | 0.69 | 0.638001 |
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Target: 5'- gGGAGAcGAAGAGG-GCcggguccccuucccgGcGGGGAGGGCc -3' miRNA: 3'- -UCUCU-CUUCUCUaCG---------------C-CCCCUCCCGc -5' |
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| 29494 | 3' | -58.5 | NC_006151.1 | + | 8559 | 0.69 | 0.633954 |
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Target: 5'- nGAGAG-AGAGcGUGCGuGuGGGuGGGUGg -3' miRNA: 3'- uCUCUCuUCUC-UACGC-C-CCCuCCCGC- -5' |
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| 29494 | 3' | -58.5 | NC_006151.1 | + | 32 | 0.69 | 0.633954 |
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Target: 5'- aAGGGGGcGGGGcuuaaaGGGGGGGGGCu -3' miRNA: 3'- -UCUCUCuUCUCuacg--CCCCCUCCCGc -5' |
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| 29494 | 3' | -58.5 | NC_006151.1 | + | 31415 | 0.69 | 0.633954 |
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Target: 5'- -aGGGGAAGGGGaagGgGGGGGAcgGGGUGc -3' miRNA: 3'- ucUCUCUUCUCUa--CgCCCCCU--CCCGC- -5' |
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| 29494 | 3' | -58.5 | NC_006151.1 | + | 32092 | 0.71 | 0.495303 |
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Target: 5'- -cAGAGAGGGGAcGCGGGGaagagacgcggaGAGGGgGa -3' miRNA: 3'- ucUCUCUUCUCUaCGCCCC------------CUCCCgC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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