Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29502 | 3' | -42.7 | NC_006151.1 | + | 53035 | 0.69 | 0.999978 |
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Target: 5'- -gGUUCGC-CgGGUUGUUGAUGUAg- -3' miRNA: 3'- ugCAAGCGuGgUUAACGAUUAUAUau -5' |
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| 29502 | 3' | -42.7 | NC_006151.1 | + | 98120 | 0.69 | 0.999969 |
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Target: 5'- cGCGcgCGCGCCGAgcccacgGUUAGUGUGc- -3' miRNA: 3'- -UGCaaGCGUGGUUaa-----CGAUUAUAUau -5' |
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| 29502 | 3' | -42.7 | NC_006151.1 | + | 13237 | 0.73 | 0.99877 |
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Target: 5'- aGCGUUCGCACUuGUUGU--AUAUAa- -3' miRNA: 3'- -UGCAAGCGUGGuUAACGauUAUAUau -5' |
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| 29502 | 3' | -42.7 | NC_006151.1 | + | 13796 | 0.74 | 0.997294 |
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Target: 5'- aGCGUUCGCACU---UGC-GGUAUAUAa -3' miRNA: 3'- -UGCAAGCGUGGuuaACGaUUAUAUAU- -5' |
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| 29502 | 3' | -42.7 | NC_006151.1 | + | 13517 | 0.74 | 0.997294 |
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Target: 5'- aGCGUUCGCACU---UGC-GGUAUAUAa -3' miRNA: 3'- -UGCAAGCGUGGuuaACGaUUAUAUAU- -5' |
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| 29502 | 3' | -42.7 | NC_006151.1 | + | 28980 | 1.08 | 0.057757 |
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Target: 5'- cACGUUCGCACCAAUUGCUAAUAUGUAc -3' miRNA: 3'- -UGCAAGCGUGGUUAACGAUUAUAUAU- -5' |
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| 29502 | 3' | -42.7 | NC_006151.1 | + | 28701 | 1.08 | 0.057757 |
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Target: 5'- cACGUUCGCACCAAUUGCUAAUAUAUAc -3' miRNA: 3'- -UGCAAGCGUGGUUAACGAUUAUAUAU- -5' |
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| 29502 | 3' | -42.7 | NC_006151.1 | + | 28422 | 1.08 | 0.057757 |
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Target: 5'- cACGUUCGCACCAAUUGCUAAUAUAUAc -3' miRNA: 3'- -UGCAAGCGUGGUUAACGAUUAUAUAU- -5' |
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| 29502 | 3' | -42.7 | NC_006151.1 | + | 79473 | 1.08 | 0.056029 |
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Target: 5'- cACGUUCGCACCAAUUGCUAAUAUAUAu -3' miRNA: 3'- -UGCAAGCGUGGUUAACGAUUAUAUAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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