Results 61 - 63 of 63 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
29504 | 3' | -55 | NC_006151.1 | + | 127707 | 0.68 | 0.884662 |
Target: 5'- gCGACGAgcucGUGgAGGCCccGGCGUCc--- -3' miRNA: 3'- -GCUGCUa---CAUgUCCGG--CCGCAGcaug -5' |
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29504 | 3' | -55 | NC_006151.1 | + | 130254 | 0.67 | 0.910602 |
Target: 5'- aGGCGcgGaAgAGGCCGGCG-CGcgGCc -3' miRNA: 3'- gCUGCuaCaUgUCCGGCCGCaGCa-UG- -5' |
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29504 | 3' | -55 | NC_006151.1 | + | 141412 | 0.68 | 0.87033 |
Target: 5'- cCGGCGAUcgGUGCGGGCgcggccCGGCG-CGaACg -3' miRNA: 3'- -GCUGCUA--CAUGUCCG------GCCGCaGCaUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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