Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 29505 | 5' | -57.2 | NC_006151.1 | + | 23196 | 1.06 | 0.003228 |
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Target: 5'- uUGUCGAGGUCGCUGGAGGUCAGCUCg -3' miRNA: 3'- -ACAGCUCCAGCGACCUCCAGUCGAGg -5' |
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| 29505 | 5' | -57.2 | NC_006151.1 | + | 23085 | 1.11 | 0.001491 |
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Target: 5'- uUGUCGAGGUCGCUGGAGGUCAGCUCCu -3' miRNA: 3'- -ACAGCUCCAGCGACCUCCAGUCGAGG- -5' |
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| 29505 | 5' | -57.2 | NC_006151.1 | + | 22900 | 0.68 | 0.753928 |
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Target: 5'- aUGUCaGAGGgCGagacGaAGGUCGGCUCCg -3' miRNA: 3'- -ACAG-CUCCaGCga--CcUCCAGUCGAGG- -5' |
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| 29505 | 5' | -57.2 | NC_006151.1 | + | 20479 | 0.66 | 0.842422 |
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Target: 5'- --cCGGGGgggCGC-GGGGGUCgucggcGGCUCUg -3' miRNA: 3'- acaGCUCCa--GCGaCCUCCAG------UCGAGG- -5' |
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| 29505 | 5' | -57.2 | NC_006151.1 | + | 19755 | 0.7 | 0.634611 |
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Target: 5'- cGUUGAGGUCGCcgucGAGGUCAuCgucgCCg -3' miRNA: 3'- aCAGCUCCAGCGac--CUCCAGUcGa---GG- -5' |
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| 29505 | 5' | -57.2 | NC_006151.1 | + | 17104 | 0.66 | 0.872996 |
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Target: 5'- cGUCGGGG-CGCcGGGGGcuccggCGGCggugCUg -3' miRNA: 3'- aCAGCUCCaGCGaCCUCCa-----GUCGa---GG- -5' |
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| 29505 | 5' | -57.2 | NC_006151.1 | + | 14419 | 0.67 | 0.808846 |
|
Target: 5'- aGUCGGGGg-GCUucccGGGUgCGGCUCCu -3' miRNA: 3'- aCAGCUCCagCGAcc--UCCA-GUCGAGG- -5' |
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| 29505 | 5' | -57.2 | NC_006151.1 | + | 13102 | 0.69 | 0.68519 |
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Target: 5'- aGggaGAGGUCGC-GGcGGGUCAcgcgcGCUCCc -3' miRNA: 3'- aCag-CUCCAGCGaCC-UCCAGU-----CGAGG- -5' |
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| 29505 | 5' | -57.2 | NC_006151.1 | + | 9334 | 0.71 | 0.583974 |
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Target: 5'- gGgagCGGGGUCGCUcGGGGGaaGGUUUCa -3' miRNA: 3'- aCa--GCUCCAGCGA-CCUCCagUCGAGG- -5' |
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| 29505 | 5' | -57.2 | NC_006151.1 | + | 5493 | 0.71 | 0.594061 |
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Target: 5'- aGUUGGGGUUGgaGGAGacggGGCUCCg -3' miRNA: 3'- aCAGCUCCAGCgaCCUCcag-UCGAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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