Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 29505 | 5' | -57.2 | NC_006151.1 | + | 27315 | 0.67 | 0.817496 |
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Target: 5'- gGUCGAGG-CGggGGAcGGggggCuGCUCCc -3' miRNA: 3'- aCAGCUCCaGCgaCCU-CCa---GuCGAGG- -5' |
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| 29505 | 5' | -57.2 | NC_006151.1 | + | 135006 | 0.67 | 0.817496 |
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Target: 5'- uUGgCGGGGgCGCgGGGGGgcagCAGCggggCCg -3' miRNA: 3'- -ACaGCUCCaGCGaCCUCCa---GUCGa---GG- -5' |
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| 29505 | 5' | -57.2 | NC_006151.1 | + | 67761 | 0.67 | 0.817496 |
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Target: 5'- cGUCGGGGUgcggcgucgCGUUGaGGGcGUCcacguGCUCCa -3' miRNA: 3'- aCAGCUCCA---------GCGAC-CUC-CAGu----CGAGG- -5' |
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| 29505 | 5' | -57.2 | NC_006151.1 | + | 57188 | 0.67 | 0.817496 |
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Target: 5'- aGUCGGGGUCGUggcGcGAGGcgaaGGC-CCg -3' miRNA: 3'- aCAGCUCCAGCGa--C-CUCCag--UCGaGG- -5' |
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| 29505 | 5' | -57.2 | NC_006151.1 | + | 14419 | 0.67 | 0.808846 |
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Target: 5'- aGUCGGGGg-GCUucccGGGUgCGGCUCCu -3' miRNA: 3'- aCAGCUCCagCGAcc--UCCA-GUCGAGG- -5' |
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| 29505 | 5' | -57.2 | NC_006151.1 | + | 62235 | 0.67 | 0.788365 |
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Target: 5'- cGUCGAGGUCGUcguguccggcuacgUGcAGGU--GCUCCc -3' miRNA: 3'- aCAGCUCCAGCG--------------ACcUCCAguCGAGG- -5' |
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| 29505 | 5' | -57.2 | NC_006151.1 | + | 88112 | 0.68 | 0.772751 |
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Target: 5'- cGUCGAGGUCcgacaGCgGGcGGgc-GCUCCg -3' miRNA: 3'- aCAGCUCCAG-----CGaCCuCCaguCGAGG- -5' |
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| 29505 | 5' | -57.2 | NC_006151.1 | + | 27460 | 0.68 | 0.769024 |
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Target: 5'- gGUCGGGGgcgggcggucgaGCgGGGGGUCGGCg-- -3' miRNA: 3'- aCAGCUCCag----------CGaCCUCCAGUCGagg -5' |
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| 29505 | 5' | -57.2 | NC_006151.1 | + | 22900 | 0.68 | 0.753928 |
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Target: 5'- aUGUCaGAGGgCGagacGaAGGUCGGCUCCg -3' miRNA: 3'- -ACAG-CUCCaGCga--CcUCCAGUCGAGG- -5' |
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| 29505 | 5' | -57.2 | NC_006151.1 | + | 23085 | 1.11 | 0.001491 |
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Target: 5'- uUGUCGAGGUCGCUGGAGGUCAGCUCCu -3' miRNA: 3'- -ACAGCUCCAGCGACCUCCAGUCGAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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