Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29505 | 5' | -57.2 | NC_006151.1 | + | 19755 | 0.7 | 0.634611 |
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Target: 5'- cGUUGAGGUCGCcgucGAGGUCAuCgucgCCg -3' miRNA: 3'- aCAGCUCCAGCGac--CUCCAGUcGa---GG- -5' |
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| 29505 | 5' | -57.2 | NC_006151.1 | + | 67830 | 0.71 | 0.604175 |
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Target: 5'- cGUCGucGUCGC-GGGGGcUCAGCagCCc -3' miRNA: 3'- aCAGCucCAGCGaCCUCC-AGUCGa-GG- -5' |
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| 29505 | 5' | -57.2 | NC_006151.1 | + | 5493 | 0.71 | 0.594061 |
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Target: 5'- aGUUGGGGUUGgaGGAGacggGGCUCCg -3' miRNA: 3'- aCAGCUCCAGCgaCCUCcag-UCGAGG- -5' |
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| 29505 | 5' | -57.2 | NC_006151.1 | + | 9334 | 0.71 | 0.583974 |
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Target: 5'- gGgagCGGGGUCGCUcGGGGGaaGGUUUCa -3' miRNA: 3'- aCa--GCUCCAGCGA-CCUCCagUCGAGG- -5' |
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| 29505 | 5' | -57.2 | NC_006151.1 | + | 103884 | 0.72 | 0.503102 |
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Target: 5'- gGUCGAGGcgcgccaccuggCGCUGGAGGUggcgcgcgCGGCcgCCg -3' miRNA: 3'- aCAGCUCCa-----------GCGACCUCCA--------GUCGa-GG- -5' |
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| 29505 | 5' | -57.2 | NC_006151.1 | + | 124588 | 0.73 | 0.485969 |
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Target: 5'- cGUCGAGGagG-UGGAGG-CGcGCUCCg -3' miRNA: 3'- aCAGCUCCagCgACCUCCaGU-CGAGG- -5' |
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| 29505 | 5' | -57.2 | NC_006151.1 | + | 133324 | 0.74 | 0.404939 |
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Target: 5'- gUGUCGAGGcgCGCcGGGcGGcCGGUUCCg -3' miRNA: 3'- -ACAGCUCCa-GCGaCCU-CCaGUCGAGG- -5' |
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| 29505 | 5' | -57.2 | NC_006151.1 | + | 119134 | 0.77 | 0.304124 |
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Target: 5'- cGUgGGGG-CGCUGGuGuUCAGCUCCa -3' miRNA: 3'- aCAgCUCCaGCGACCuCcAGUCGAGG- -5' |
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| 29505 | 5' | -57.2 | NC_006151.1 | + | 23196 | 1.06 | 0.003228 |
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Target: 5'- uUGUCGAGGUCGCUGGAGGUCAGCUCg -3' miRNA: 3'- -ACAGCUCCAGCGACCUCCAGUCGAGg -5' |
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| 29505 | 5' | -57.2 | NC_006151.1 | + | 23085 | 1.11 | 0.001491 |
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Target: 5'- uUGUCGAGGUCGCUGGAGGUCAGCUCCu -3' miRNA: 3'- -ACAGCUCCAGCGACCUCCAGUCGAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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