miRNA: 3'- -GUCCgCGGCCA-------------UGAC-----CGGGAGCaaCU- -5'
miRNA: 3'- gUCCGCGGCCaugaCCGGGaGCaaCU- -5'
miRNA: 3'- -GUCCGCGGC-----------CAUgACCGgGAGCaacu -5'
Results 41 - 43 of 43 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29507 | 3' | -59.3 | NC_006151.1 | + | 64772 | 0.66 | 0.772994 |
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Target: 5'- gCGGGgGCUGGUugccggggcgcgaGCUGgccacGCCCUCGcgGAa -3' miRNA: 3'- -GUCCgCGGCCA-------------UGAC-----CGGGAGCaaCU- -5' |
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| 29507 | 3' | -59.3 | NC_006151.1 | + | 17108 | 0.66 | 0.773914 |
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Target: 5'- gGGGCGCCGGg---GGCUC-CGgcGGc -3' miRNA: 3'- gUCCGCGGCCaugaCCGGGaGCaaCU- -5' |
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| 29507 | 3' | -59.3 | NC_006151.1 | + | 100225 | 0.66 | 0.773914 |
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Target: 5'- gCGGGCGCCGuggccgagcgcGUGgUGGCgCUCGa--- -3' miRNA: 3'- -GUCCGCGGC-----------CAUgACCGgGAGCaacu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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