miRNA: 3'- aaCGUACGAGCGGgGUGcACCG-GCGG- -5'
miRNA: 3'- aACGUACGa--GCGGGGUGCACCGGcGG- -5'
miRNA: 3'- aaCGU--ACGAgCGGGGUGCAC-CGGCGG- -5'
miRNA: 3'- -AACGUACGAGCGGGGUGCACCGGCGG- -5'
Results 181 - 184 of 184 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29515 | 3' | -61.4 | NC_006151.1 | + | 85345 | 0.8 | 0.089399 |
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Target: 5'- -aGCGUGCUCGCCgCGC-UGGCgCGCCu -3' miRNA: 3'- aaCGUACGAGCGGgGUGcACCG-GCGG- -5' |
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| 29515 | 3' | -61.4 | NC_006151.1 | + | 73951 | 0.81 | 0.074445 |
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Target: 5'- gUGCGUGCgcaUGUCCCGCGUGGCCaCCg -3' miRNA: 3'- aACGUACGa--GCGGGGUGCACCGGcGG- -5' |
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| 29515 | 3' | -61.4 | NC_006151.1 | + | 114115 | 0.82 | 0.060292 |
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Target: 5'- -gGCGgcUGCU-GCCCCACGUGcGCCGCCg -3' miRNA: 3'- aaCGU--ACGAgCGGGGUGCAC-CGGCGG- -5' |
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| 29515 | 3' | -61.4 | NC_006151.1 | + | 7256 | 1.08 | 0.000904 |
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Target: 5'- uUUGCAUGCUCGCCCCACGUGGCCGCCc -3' miRNA: 3'- -AACGUACGAGCGGGGUGCACCGGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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