miRNA: 3'- aaCGuaCGAGCGGgGuGCA-CCGGCGG- -5'
miRNA: 3'- aaCGUaCGAgC-GGGGU-GCACCGGcGG- -5'
miRNA: 3'- aaCGUa--CGaGCGGGgUGCaCCGGCGG- -5'
miRNA: 3'- aaCGUa-CGa--GCGGGGUgCACCG--GCGG- -5'
Results 181 - 184 of 184 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 29515 | 3' | -61.4 | NC_006151.1 | + | 139923 | 0.73 | 0.255253 |
|
Target: 5'- -cGCggGCUCGUCgC-CGUgGGCCGCCg -3' miRNA: 3'- aaCGuaCGAGCGGgGuGCA-CCGGCGG- -5' |
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| 29515 | 3' | -61.4 | NC_006151.1 | + | 140185 | 0.71 | 0.321048 |
|
Target: 5'- -cGCAcGCUgGaCCCCAuCGUGGCCaCCg -3' miRNA: 3'- aaCGUaCGAgC-GGGGU-GCACCGGcGG- -5' |
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| 29515 | 3' | -61.4 | NC_006151.1 | + | 141597 | 0.69 | 0.459811 |
|
Target: 5'- -gGCGagaGC-CGCCCgGCGaGGCUGCCg -3' miRNA: 3'- aaCGUa--CGaGCGGGgUGCaCCGGCGG- -5' |
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| 29515 | 3' | -61.4 | NC_006151.1 | + | 141752 | 0.7 | 0.390446 |
|
Target: 5'- -cGCGggGCgcgCGCCCCG-GUGGCguCGCCg -3' miRNA: 3'- aaCGUa-CGa--GCGGGGUgCACCG--GCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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