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miRNA: 3'- gguaCCGCAGGgGCuaCaCGCCGUCg -5'
miRNA: 3'- -GGuACCGC-----AGGgGCUa---CACG-CCGUCG- -5'
miRNA: 3'- -GGUa----CCGCagGGGCuaCaCGCCGUCG- -5'
miRNA: 3'- -GGUa-CCGCa-------------GGG-GCuaCA---CGCCGUCG- -5'
Results 101 - 104 of 104 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio#
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P value |
| Predicted miRNA align pattern | |||||||
| 29517 | 3' | -58.4 | NC_006151.1 | + | 122454 | 0.66 | 0.817095 |
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Target: 5'- ---cGGCGUCCaCGgcG-GCGGCGGg -3' miRNA: 3'- gguaCCGCAGGgGCuaCaCGCCGUCg -5' |
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| 29517 | 3' | -58.4 | NC_006151.1 | + | 123610 | 0.66 | 0.817095 |
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Target: 5'- gCC-UGGCGauauaUCCgCGAgcugGUGCuGGCGGUu -3' miRNA: 3'- -GGuACCGC-----AGGgGCUa---CACG-CCGUCG- -5' |
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| 29517 | 3' | -58.4 | NC_006151.1 | + | 36625 | 0.66 | 0.817095 |
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Target: 5'- gCCGgucucGGCG--CCCGgcG-GCGGCGGCg -3' miRNA: 3'- -GGUa----CCGCagGGGCuaCaCGCCGUCG- -5' |
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| 29517 | 3' | -58.4 | NC_006151.1 | + | 30919 | 0.66 | 0.836953 |
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Target: 5'- cCCGcgGGCGgcccgcgcggaucgCCCgCGcgGUaucGCGGCGGCc -3' miRNA: 3'- -GGUa-CCGCa-------------GGG-GCuaCA---CGCCGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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