miRNA: 3'- -GGuaCCGCa----GGGGCuacaCGC-CGUCG- -5'
miRNA: 3'- -GGUACC-GCAGGGGCuaCaCgCCGuCG- -5'
miRNA: 3'- gguaCCGca--GGGGCuaCACG-CCGUCg -5'
miRNA: 3'- -GGUaCCGCaGGGGCuacaCGCCGUCG- -5'
Results 101 - 104 of 104 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 29517 | 3' | -58.4 | NC_006151.1 | + | 3678 | 0.69 | 0.646438 |
|
Target: 5'- cCCggGGCGgcgggCCCCGg---GCGcGCGGCg -3' miRNA: 3'- -GGuaCCGCa----GGGGCuacaCGC-CGUCG- -5' |
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| 29517 | 3' | -58.4 | NC_006151.1 | + | 3621 | 0.72 | 0.461623 |
|
Target: 5'- gCCGUGGcCGUCCCCGcgGaG-GGCcGCg -3' miRNA: 3'- -GGUACC-GCAGGGGCuaCaCgCCGuCG- -5' |
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| 29517 | 3' | -58.4 | NC_006151.1 | + | 2689 | 0.67 | 0.763621 |
|
Target: 5'- ---cGGCucggCCCCGggGUGCaGGCGGg -3' miRNA: 3'- gguaCCGca--GGGGCuaCACG-CCGUCg -5' |
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| 29517 | 3' | -58.4 | NC_006151.1 | + | 2219 | 0.74 | 0.360039 |
|
Target: 5'- uCCAcGGCGcCCCCG----GCGGCGGCg -3' miRNA: 3'- -GGUaCCGCaGGGGCuacaCGCCGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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