miRNA: 3'- gGUACCGca---GGGGCUACACG------------CCguCG- -5'
miRNA: 3'- -GGUACCgCAG--------GGGC-------------UACa---CGCCGUCG- -5'
miRNA: 3'- gguacCGCAGG---GGCuaCaCGCCGUCG- -5'
miRNA: 3'- -GGUACCGCaGG-GGCUACaCGccGUCG- -5'
Results 101 - 104 of 104 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 29517 | 3' | -58.4 | NC_006151.1 | + | 19938 | 0.67 | 0.763621 |
|
Target: 5'- gCAUGGUcucaaCCCCGGUGUGUcgagacucgcggGGguGCc -3' miRNA: 3'- gGUACCGca---GGGGCUACACG------------CCguCG- -5' |
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| 29517 | 3' | -58.4 | NC_006151.1 | + | 17372 | 0.67 | 0.762691 |
|
Target: 5'- gCCGUGGgGUCggaccgugUCCGcacgacggggcgcGUGgcgcGCGGCAGCa -3' miRNA: 3'- -GGUACCgCAG--------GGGC-------------UACa---CGCCGUCG- -5' |
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| 29517 | 3' | -58.4 | NC_006151.1 | + | 96702 | 0.67 | 0.754267 |
|
Target: 5'- ----uGCGUCCgcgCCGggG-GCGGCGGCg -3' miRNA: 3'- gguacCGCAGG---GGCuaCaCGCCGUCG- -5' |
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| 29517 | 3' | -58.4 | NC_006151.1 | + | 38184 | 0.68 | 0.725617 |
|
Target: 5'- aCCGUGGCG-CCgCUGGUGcGCuaCAGCg -3' miRNA: 3'- -GGUACCGCaGG-GGCUACaCGccGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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