miRNA display CGI


Results 1 - 8 of 8 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
29522 3' -52.5 NC_006151.1 + 72397 0.66 0.981104
Target:  5'- gACCcg-GCCGUGGUCUUCuaccaCCacGGc -3'
miRNA:   3'- -UGGuaaCGGCGCCAGAAGua---GGaaCC- -5'
29522 3' -52.5 NC_006151.1 + 60609 0.66 0.972152
Target:  5'- uGCCuccGUCGCGGUCUcCAUCUggccgcggcgcgggUGGg -3'
miRNA:   3'- -UGGuaaCGGCGCCAGAaGUAGGa-------------ACC- -5'
29522 3' -52.5 NC_006151.1 + 131167 0.67 0.967956
Target:  5'- cACCAUcGCCGCGGUCcgagcUCggCCa-GGa -3'
miRNA:   3'- -UGGUAaCGGCGCCAGa----AGuaGGaaCC- -5'
29522 3' -52.5 NC_006151.1 + 91928 0.67 0.964358
Target:  5'- cACCAgcGCCGUcauggucGGUCUcgcUCGgucCCUUGGu -3'
miRNA:   3'- -UGGUaaCGGCG-------CCAGA---AGUa--GGAACC- -5'
29522 3' -52.5 NC_006151.1 + 9990 0.67 0.957493
Target:  5'- gGCCcgcgaggcgGCCGCGGgcgUCA-CCUUGGc -3'
miRNA:   3'- -UGGuaa------CGGCGCCagaAGUaGGAACC- -5'
29522 3' -52.5 NC_006151.1 + 105988 0.68 0.942127
Target:  5'- uACCGc-GCCGCGGccgggcccgugcccgUCUUCAUCCcggagaUGGg -3'
miRNA:   3'- -UGGUaaCGGCGCC---------------AGAAGUAGGa-----ACC- -5'
29522 3' -52.5 NC_006151.1 + 106528 0.68 0.940223
Target:  5'- cGCCGcgGCCGCGG-CUUCAgcagcUCCa--- -3'
miRNA:   3'- -UGGUaaCGGCGCCaGAAGU-----AGGaacc -5'
29522 3' -52.5 NC_006151.1 + 41220 0.7 0.864616
Target:  5'- cGCCGUcGCCGCGcUCUUCGagcccaUCCU-GGg -3'
miRNA:   3'- -UGGUAaCGGCGCcAGAAGU------AGGAaCC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.