Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29522 | 3' | -52.5 | NC_006151.1 | + | 72397 | 0.66 | 0.981104 |
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Target: 5'- gACCcg-GCCGUGGUCUUCuaccaCCacGGc -3' miRNA: 3'- -UGGuaaCGGCGCCAGAAGua---GGaaCC- -5' |
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| 29522 | 3' | -52.5 | NC_006151.1 | + | 60609 | 0.66 | 0.972152 |
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Target: 5'- uGCCuccGUCGCGGUCUcCAUCUggccgcggcgcgggUGGg -3' miRNA: 3'- -UGGuaaCGGCGCCAGAaGUAGGa-------------ACC- -5' |
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| 29522 | 3' | -52.5 | NC_006151.1 | + | 131167 | 0.67 | 0.967956 |
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Target: 5'- cACCAUcGCCGCGGUCcgagcUCggCCa-GGa -3' miRNA: 3'- -UGGUAaCGGCGCCAGa----AGuaGGaaCC- -5' |
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| 29522 | 3' | -52.5 | NC_006151.1 | + | 91928 | 0.67 | 0.964358 |
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Target: 5'- cACCAgcGCCGUcauggucGGUCUcgcUCGgucCCUUGGu -3' miRNA: 3'- -UGGUaaCGGCG-------CCAGA---AGUa--GGAACC- -5' |
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| 29522 | 3' | -52.5 | NC_006151.1 | + | 9990 | 0.67 | 0.957493 |
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Target: 5'- gGCCcgcgaggcgGCCGCGGgcgUCA-CCUUGGc -3' miRNA: 3'- -UGGuaa------CGGCGCCagaAGUaGGAACC- -5' |
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| 29522 | 3' | -52.5 | NC_006151.1 | + | 105988 | 0.68 | 0.942127 |
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Target: 5'- uACCGc-GCCGCGGccgggcccgugcccgUCUUCAUCCcggagaUGGg -3' miRNA: 3'- -UGGUaaCGGCGCC---------------AGAAGUAGGa-----ACC- -5' |
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| 29522 | 3' | -52.5 | NC_006151.1 | + | 106528 | 0.68 | 0.940223 |
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Target: 5'- cGCCGcgGCCGCGG-CUUCAgcagcUCCa--- -3' miRNA: 3'- -UGGUaaCGGCGCCaGAAGU-----AGGaacc -5' |
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| 29522 | 3' | -52.5 | NC_006151.1 | + | 41220 | 0.7 | 0.864616 |
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Target: 5'- cGCCGUcGCCGCGcUCUUCGagcccaUCCU-GGg -3' miRNA: 3'- -UGGUAaCGGCGCcAGAAGU------AGGAaCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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