miRNA: 3'- -UGGGa-UCuuCCGC-UCGGCGCuaCGac -5'
miRNA: 3'- uGGGAUCuuCCGCUCGG-CGc------UACGAC- -5'
miRNA: 3'- -UGGGAUCuu---CCGCUCGGCG-CUACGac -5'
miRNA: 3'- ugGGAUCU-----UCCGCuCGGCGCU--ACGAC- -5'
Results 41 - 44 of 44 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29522 | 5' | -57.9 | NC_006151.1 | + | 28305 | 0.73 | 0.409722 |
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Target: 5'- cGCCCgaAGgcGGCGaAGCCGCGcgGCa- -3' miRNA: 3'- -UGGGa-UCuuCCGC-UCGGCGCuaCGac -5' |
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| 29522 | 5' | -57.9 | NC_006151.1 | + | 36418 | 0.73 | 0.401118 |
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Target: 5'- cCCCUGGccGGCGAGCCgGCcugccucGUGCUGg -3' miRNA: 3'- uGGGAUCuuCCGCUCGG-CGc------UACGAC- -5' |
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| 29522 | 5' | -57.9 | NC_006151.1 | + | 22323 | 0.73 | 0.392629 |
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Target: 5'- cAUCCUGGccgacGGCGGGCCGCuGGUGCa- -3' miRNA: 3'- -UGGGAUCuu---CCGCUCGGCG-CUACGac -5' |
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| 29522 | 5' | -57.9 | NC_006151.1 | + | 94302 | 0.74 | 0.376006 |
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Target: 5'- -gCCUGGAggccuGGGCGcGCCGCGGggUGCUGc -3' miRNA: 3'- ugGGAUCU-----UCCGCuCGGCGCU--ACGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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