Results 161 - 167 of 167 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 29523 | 3' | -55.5 | NC_006151.1 | + | 139930 | 0.67 | 0.8614 |
|
Target: 5'- aGCAGcGCGggaGCggggUCCGgAGCGGGCCCGa -3' miRNA: 3'- -CGUC-UGUa--CGg---AGGUgUUGCUCGGGC- -5' |
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| 29523 | 3' | -55.5 | NC_006151.1 | + | 140615 | 0.66 | 0.909671 |
|
Target: 5'- cGUAGACGUGguaguCCcCCACGGCcGGGCCg- -3' miRNA: 3'- -CGUCUGUAC-----GGaGGUGUUG-CUCGGgc -5' |
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| 29523 | 3' | -55.5 | NC_006151.1 | + | 140757 | 0.73 | 0.53344 |
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Target: 5'- uGCAGGCGggcgaaggagGCCUCCACGcgggcgaagcagGcCGGGCCCa -3' miRNA: 3'- -CGUCUGUa---------CGGAGGUGU------------U-GCUCGGGc -5' |
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| 29523 | 3' | -55.5 | NC_006151.1 | + | 141259 | 0.73 | 0.553494 |
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Target: 5'- cGCGGGCcgguggGUCUCCACGGCGccCCCGg -3' miRNA: 3'- -CGUCUGua----CGGAGGUGUUGCucGGGC- -5' |
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| 29523 | 3' | -55.5 | NC_006151.1 | + | 141381 | 0.75 | 0.456271 |
|
Target: 5'- -gAGAUG-GCCUCCACcuuGAUGGGCCCGa -3' miRNA: 3'- cgUCUGUaCGGAGGUG---UUGCUCGGGC- -5' |
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| 29523 | 3' | -55.5 | NC_006151.1 | + | 141794 | 0.69 | 0.78471 |
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Target: 5'- -gGGACGauCCUCCGCcgcCGAGCCCu -3' miRNA: 3'- cgUCUGUacGGAGGUGuu-GCUCGGGc -5' |
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| 29523 | 3' | -55.5 | NC_006151.1 | + | 142732 | 0.76 | 0.377193 |
|
Target: 5'- cGCGGGCGgggGCUUCCGCuccGCGGcGCCCGc -3' miRNA: 3'- -CGUCUGUa--CGGAGGUGu--UGCU-CGGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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