miRNA: 3'- uUCUCCUGGGUCaGC-CCGaa----CCGGUG- -5'
miRNA: 3'- uUCU-CCUGGGUCa-------GCCCGaa-------CCGGUG- -5'
miRNA: 3'- uucUCCUGGgUCaGCCCG----AACCGGUG- -5'
miRNA: 3'- uuCUCCUGgGUCagCCCGaa------CCGGUG- -5'
miRNA: 3'- uucUCCUGGGUCAGCCCGaaCCGGuG- -5'
Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 29526 | 5' | -60 | NC_006151.1 | + | 131108 | 0.76 | 0.203416 |
|
Target: 5'- cGGGGGGCCCGGcCGcGGCgcgggaGGCCGCg -3' miRNA: 3'- uUCUCCUGGGUCaGC-CCGaa----CCGGUG- -5' |
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| 29526 | 5' | -60 | NC_006151.1 | + | 133146 | 0.66 | 0.707883 |
|
Target: 5'- cAGAgGGACUCGGcggcggcgCGGGCgugggccucGGCCACg -3' miRNA: 3'- uUCU-CCUGGGUCa-------GCCCGaa-------CCGGUG- -5' |
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| 29526 | 5' | -60 | NC_006151.1 | + | 133400 | 0.72 | 0.365119 |
|
Target: 5'- --cGGGGCCgAG-CGGGCggucUUGGCCGCg -3' miRNA: 3'- uucUCCUGGgUCaGCCCG----AACCGGUG- -5' |
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| 29526 | 5' | -60 | NC_006151.1 | + | 135010 | 0.66 | 0.677787 |
|
Target: 5'- -cGGGGGCgCGGggGGGCagcagcggGGCCGCg -3' miRNA: 3'- uuCUCCUGgGUCagCCCGaa------CCGGUG- -5' |
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| 29526 | 5' | -60 | NC_006151.1 | + | 141975 | 0.72 | 0.357195 |
|
Target: 5'- --cGGGAUCCGGUCGGGC--GGCC-Cg -3' miRNA: 3'- uucUCCUGGGUCAGCCCGaaCCGGuG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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