Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 29526 | 5' | -60 | NC_006151.1 | + | 18764 | 0.67 | 0.596501 |
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Target: 5'- -cGGGGuGCCCGGggccCGGGCcggGGCCGu -3' miRNA: 3'- uuCUCC-UGGGUCa---GCCCGaa-CCGGUg -5' |
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| 29526 | 5' | -60 | NC_006151.1 | + | 66858 | 0.67 | 0.60665 |
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Target: 5'- cGAGAGGGCCgGGgCGGcGCgaagGGCgCGCc -3' miRNA: 3'- -UUCUCCUGGgUCaGCC-CGaa--CCG-GUG- -5' |
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| 29526 | 5' | -60 | NC_006151.1 | + | 79208 | 0.67 | 0.60665 |
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Target: 5'- aGAGGGGAuuCCCGGUCGcucGGCcccaGCCGCg -3' miRNA: 3'- -UUCUCCU--GGGUCAGC---CCGaac-CGGUG- -5' |
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| 29526 | 5' | -60 | NC_006151.1 | + | 102283 | 0.67 | 0.60665 |
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Target: 5'- cGGAGcgcGGCCCGGcCGGGgacuuugUGGCCGCc -3' miRNA: 3'- uUCUC---CUGGGUCaGCCCga-----ACCGGUG- -5' |
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| 29526 | 5' | -60 | NC_006151.1 | + | 77238 | 0.66 | 0.707883 |
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Target: 5'- cGGGAGGcgcccCCCGG-CGGGUUuaaaggagUGGUCGCg -3' miRNA: 3'- -UUCUCCu----GGGUCaGCCCGA--------ACCGGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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