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miRNA: 3'- aUGCGc------CUCGaGUACCugCGCGAGCU- -5'
miRNA: 3'- aUGCGCCUCgaGuaCcUGCGCGAGCU- -5'
miRNA: 3'- aUGCGCCU---CGAGuACCUGCGCGaGCU- -5'
miRNA: 3'- -AUGCGCCUCGaGUACcUGCGCGAGCu -5'
miRNA: 3'- aUGCGCCUCGA---------------GuaCCUGCGCGAGCU- -5'
Results 101 - 105 of 105 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio#
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P value |
| Predicted miRNA align pattern | |||||||
| 29529 | 5' | -58.1 | NC_006151.1 | + | 100228 | 0.77 | 0.229857 |
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Target: 5'- gGCGCcguggccGAGCgCGUGGugGCGCUCGAg -3' miRNA: 3'- aUGCGc------CUCGaGUACCugCGCGAGCU- -5' |
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| 29529 | 5' | -58.1 | NC_006151.1 | + | 138144 | 0.79 | 0.188849 |
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Target: 5'- gACGCGGAGgaCcgGcGCGCGCUCGAg -3' miRNA: 3'- aUGCGCCUCgaGuaCcUGCGCGAGCU- -5' |
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| 29529 | 5' | -58.1 | NC_006151.1 | + | 122513 | 0.8 | 0.154415 |
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Target: 5'- cGCGCGGAcgcGCUCcUGGACGCGCaCGAc -3' miRNA: 3'- aUGCGCCU---CGAGuACCUGCGCGaGCU- -5' |
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| 29529 | 5' | -58.1 | NC_006151.1 | + | 104781 | 0.83 | 0.09947 |
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Target: 5'- gUACGCGGAGC-CAUGcGCGCGCUCGu -3' miRNA: 3'- -AUGCGCCUCGaGUACcUGCGCGAGCu -5' |
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| 29529 | 5' | -58.1 | NC_006151.1 | + | 81752 | 0.86 | 0.06577 |
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Target: 5'- gGCGCGGAGCUgguccgcgggcgccaCcgGGACGCGCUCGGc -3' miRNA: 3'- aUGCGCCUCGA---------------GuaCCUGCGCGAGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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