Results 101 - 105 of 105 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29529 | 5' | -58.1 | NC_006151.1 | + | 111549 | 0.66 | 0.811487 |
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Target: 5'- gGCGCGcAGCggccgCGUGuccacgcccccGGCGCGCUUGAu -3' miRNA: 3'- aUGCGCcUCGa----GUAC-----------CUGCGCGAGCU- -5' |
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| 29529 | 5' | -58.1 | NC_006151.1 | + | 83148 | 0.66 | 0.814956 |
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Target: 5'- cGCGCcaaagaggguguccuGGAGCUCggGGucguCGCGCgggCGGc -3' miRNA: 3'- aUGCG---------------CCUCGAGuaCCu---GCGCGa--GCU- -5' |
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| 29529 | 5' | -58.1 | NC_006151.1 | + | 102116 | 0.66 | 0.820111 |
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Target: 5'- gUGCGCGGGccggagccuGUUCcccGGGCGCGC-CGGg -3' miRNA: 3'- -AUGCGCCU---------CGAGua-CCUGCGCGaGCU- -5' |
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| 29529 | 5' | -58.1 | NC_006151.1 | + | 99698 | 0.66 | 0.820111 |
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Target: 5'- gGCGCcggGGAGaCgccCAUGGAgGCGC-CGAc -3' miRNA: 3'- aUGCG---CCUC-Ga--GUACCUgCGCGaGCU- -5' |
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| 29529 | 5' | -58.1 | NC_006151.1 | + | 48948 | 0.66 | 0.820111 |
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Target: 5'- cGCGCGcGAGgaCGuguucgccUGGACGCGcCUCa- -3' miRNA: 3'- aUGCGC-CUCgaGU--------ACCUGCGC-GAGcu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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